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anti-Desmoglein 2 mouse monoclonal, 10G11, supernatant

The anti-desmoglein 2 antibody recognizes desmoglein 2 present in all epithelia, in heart muscle and lymphatic follicles.
Cat. No.: 65159
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 10G11
Immunogen Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (negative with mouse, rat)

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use (preincubation (directly after fixation) with 0.05 - 0.2% Triton X100, for 5-10 min, depending on tissue type, is recommended)
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Antibody recognizes desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.
Polypeptide reacting: desmoglein 2 (1069aa; calculated mol. wt. 116,760).

Reactivity on cultured cell lines: CaCo-2, HaCaT, MCF-7.

Positive control: CaCo-2, HaCaT

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