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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | G96 |
| Immunogen | recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain) |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 100 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human (negative with mouse, rat) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | assay dependent |
| Immunohistochemistry (IHC) - frozen | ready-to-use (for better resolution preincubation (directliy after fixation) with 0.05 - 0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended) |
| Immunohistochemistry (IHC) - paraffin | ready-to-use (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
Antibody recognizes desmoglein 2 present in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.
Polypeptide reacting: Desmoglein 2 (1069aa; calculated mol. wt. 116,760).
Recommended as positive control: CaCo-2, HaCaT.
Reactivities on Cultured cell lines: Many, including CaCo-2, HaCaT, MCF-7.
Reference
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human
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IHC (frozen)
IHC (frozen)
human
IHC (paraffin)
