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Product description
| Host | rabbit |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | recombinant peptide (extracellular repeat domain E2 of human Desmoglein 2) |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| UniprotID | Q14126 |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 60 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:100 (preincubation (directly after fixation with –20°C methanol) with 0.05 - 0.5% Triton X-100, for 5-10 min, depending on tissue type, recommended) |
| Western Blot (WB) | 1:3,000 |
Background
Details
Antibody recognizes desmoglein 2 present in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.
Polypeptide reacting: Desmoglein 2 (1069aa; calculated mol. wt. 116,760)
Reactivity on cultured cell lines: Many, including CaCo-2, HaCaT, MCF-7.
Reference
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