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IHC of human oral mucosa (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human oral mucosa (courtesy of J.Heß, University Hospital Heidelberg)

anti-Desmoglein 3 mouse monoclonal, Dsg-G194, supernatant

The anti-desmoglein 3 antibody stains basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. Pattern corresponds to those obtained with pemphigus vulgaris patient sera.
Cat. No.: 651112
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€211.00
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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Dsg-G194
Immunogen recombinant human polypeptide Desmoglein 3 (C-terminal aa 920-940 VSIPDPLQHGNYLVTETYSAS)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen undiluted - 1:50 (for better resolution preincubation (directly after fixation) with 0.05 - 0.2% Triton X-100 for 5 - 10 min, depending on tissue type, is recommended)
Immunohistochemistry (IHC) - paraffin undiluted - 1:50 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

In immunoblots of cytoskeletal preparations from human epidermis the antibody reacts specifically with a 140 kD polypeptide typical for Desmoglein 3. In immunolocalization studies the mab shows typical staining of basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus vulgaris patient sera.
Cross-reaction with Desmogleins 1 and 2 was not detected.

Reference

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