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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| 651112S |
anti-Desmoglein 3 mouse monoclonal, Dsg-G194 |
supernatant, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
| 70032 |
anti-Desmosome sample set 3 |
600 µl each antibody |
$230.00
|
|
| 70033 |
anti-Desmoglein 1-4 sample set |
600 µl each antibody |
$184.00
|
Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | Dsg-G194 |
| Immunogen | recombinant human polypeptide Desmoglein 3 (C-terminal aa 920-940 VSIPDPLQHGNYLVTETYSAS) |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 5000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | undiluted - 1:50 (for better resolution preincubation (directly after fixation) with 0.05 - 0.2% Triton X-100 for 5 - 10 min, depending on tissue type, is recommended) |
| Immunohistochemistry (IHC) - paraffin | undiluted - 1:50 (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
In immunoblots of cytoskeletal preparations from human epidermis the antibody reacts specifically with a 140 kD polypeptide typical for Desmoglein 3. In immunolocalization studies the mab shows typical staining of basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus vulgaris patient sera.
Cross-reaction with Desmogleins 1 and 2 was not detected.
