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anti-Desmoplakin 1/2 mouse monoclonal, DP 1 + 2-2.15, lyophilized, purified

The anti-desmoplakin 1/2 antibody decorates desmoplakin 1 and 2 in desmosomal complexes. The anti-desmoplakin 1/2 antibody is useful for staining of primary and metastatic carcinoma.
Cat. No.: 61003
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€211.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DP1 + 2-2.15
Immunogen bovine desmoplakin 1&2
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) assay dependent

Background

Details

DP1&2-2.15 shows distinct punctate membrane staining of different epithelia. Tumors specifically detected: Primary and metastatic carcinoma and meningioma. Polypeptides reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000)

Tested cultured cell lines: Several human carcinoma cell lines: MCF-7, A-431; rat MH1C1 cell line; bovine cells: MDBK, BMGE

Reference

Reference

PublicationSpeciesApplication
Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750–1758 (2012). mouse IHC (frozen)
Miravet, S. et al. Tyrosine Phosphorylation of Plakoglobin Causes Contrary Effects on Its Association with Desmosomes and Adherens Junction Components and Modulates ␤-Catenin-Mediated Transcription. Mol. Cell. Biol. 23, 7391–7402 (2003). human WB
Bosch, F. X., Andl, C., Abel, U. & Kartenbeck, J. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: A comparison of E-cadherin with desmosomal components. Int. J. Cancer 114, 779–790 (2005). human IHC (frozen)
Weiske, J. et al. The Fate of Desmosomal Proteins in Apoptotic Cells. J. Biol. Chem. 276, 41175–41181 (2001). human WB, ICC-IF
Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209–222 (2000). human ICC-IF
Moll, I., Houdek, P., Schäfer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437–46 (1999). human IHC (frozen)
Molj, I., Kurzcn, H., Langbein, L., Erner, W. & Franket, W. The Distribution of the DesmosoInal Protein, Plakophilin 1, in Human Skin and Skin Tumors. J. Invest. Dermatol. 108, 139–146 (1997). human IHC (frozen)
Owaribe, K., Nishizawa, Y. & Franke, W. W. Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Res. 192, 622–30 (1991). bovine IHC (frozen)
Owaribe, K. et al. The hemidesmosomal plaque. I. Characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation. 45, 207–20 (1990). rat, bovine IHC (frozen)
Rungger-Brandle, E., Achtstatter, T. & Franke, W. W. An epithelium-type cytoskeleton in a glial cell: Astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes. J. Cell Biol. 109, 705–716 (1989). R. ridibunda IHC (frozen)
Moll, I; Heid, H; Moll, R. Cytokeratin analysis of pilomatrixoma: changes in cytokeratin-type expression during differentiation. J. Invest. Dermatol. 91, 251–7 (1988). human IHC (frozen)
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987). human IHC (frozen)
Dockhorn-Dworniczak, B. et al. Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. Differentiation. 35, 53–71 (1987). human IHC (frozen)
Achtstätter, T. et al. Expression of glial filament protein (GFP) in nerve sheaths and non-neural cells re-examined using monoclonal antibodies, with special emphasis on the co-expression of GFP and cytokeratins in epithelial cells of human salivary gland and pleomorphic. Differentiation 31, 206–227 (1986). human IHC (frozen)
Cowln, P., Kapprell, H.-P. & Franke, W. W. The Complement of Desmosomal Plaque Proteins in Different Cell Types. J. Cell Biol. 101, 1442–1454 (1985) human, bovine IHC (frozen), IEM

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