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anti-Desmoplakin 1/2 mouse monoclonal, DP-2.15 + DP-2.17 + DP-2.20, supernatant

The desmoplakin antibody cocktail shows distinct punctate membrane staining of different epithelia.
Cat. No.: 65146
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€146.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DP1&2-2.15; DP1-2.17; DP1&2-2.20
Immunogen bovine desmoplakins 1&2
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, dog, human, mouse, pig, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The antibody cocktail shows distinct punctate membrane staining of different epithelia and the aerea composite of the myocard. Tumors specifically detected: Primary and metastatic cancer; meningioma.

Polypeptides reacting: Desmoplakins 1 and 2 (Mr 250.000 and 215.000)

Tested cultured cell lines: Several human carcinoma cell lines: MCF-7, A-431; rat MH1C1 cell line; bovine cells: BMGE, MDBK; MDCK (dog)

Reference

Reference

PublicationSpeciesApplication
Kant, S., Holthöfer, B., Magin, T. M., Krusche, C. A. & Leube, R. E. Desmoglein 2-Dependent Arrhythmogenic Cardiomyopathy Is Caused by a Loss of Adhesive Function. Circ. Cardiovasc. Genet. 8, 553–63 (2015). mouse IHC (paraffin)
Fischer-Kešo, R. et al. Plakophilins 1 and 3 bind to FXR1 and thereby influence the mRNA stability of desmosomal proteins. Mol. Cell. Biol. 34, 4244–56 (2014). human WB, IP
Franke, W. W. et al. Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology. Cell Tissue Res. 357, 159–72 (2014). human, pig IHC (frozen), ICC-IF
Langbein, L. et al. New facets of keratin K77: Interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793–812 (2013). human IHC (frozen)
Ibi, M. et al. Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. J Cell Sci 124, 857–864 (2011). mouse ICC-IF
Langbein, L., Eckhart, L., Rogers, M. A., Praetzel-Wunder, S. & Schweizer, J. Against the rules: Human keratin K80 - Two functional alternative splice variants, K80 and K80.1, with special cellular localization in a wide range of epithelia. J. Biol. Chem. 285, 36909–36921 (2010). human IHC (frozen), IEM
Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells. Int. J. cancer 125, 2036–48 (2009). human WB
Walter, B., Schlechter, T., Hergt, M., Berger, I. & Hofmann, I. Differential expression pattern of protein ARVCF in nephron segments of human and mouse kidney. Histochem. Cell Biol. 130, 943–956 (2008). human IHC (frozen)
Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates – IV: Coalescence and amalgamation of desmosomal and adhaerens junction components – Late processes in mammalian heart development. Eur. J. Cell Biol. 86, 377–391 (2007). mouse IHC (frozen), IEM
Bortfeld, M. et al. Human multidrug resistance protein 8 (MRP8/ABCC11), an apical efflux pump for steroid sulfates, is an axonal protein of the CNS and peripheral nervous system. Neuroscience 137, 1247–1257 (2006). dog ICC-IF
Borrmann, C. M. et al. The area composita of adhering junctions connecting heart muscle cells of vertebrates. II. Colocalizations of desmosomal and fascia adhaerens molecules in the intercalated disk. Eur. J. Cell Biol. 85, 469–485 (2006). human, mouse, rat, bovine, eel, carp, xenopus WB, IHC (frozen)
Franke, W. W., Borrmann, C. M., Grund, C. & Pieperhoff, S. The area composita of adhering junctions connecting heart muscle cells of vertebrates. I. Molecular definition in intercalated disks of cardiomyocytes by immunoelectron microscopy of desmosomal proteins. European Journal of Cell Biology 85, 69–82 (2006). human, mouse, rat IEM
Silberberg, M., Charron, A. J., Bacallao, R. & Wandinger-Ness, A. Mispolarization of Desmosomal Proteins and Altered Cell Adhesion in Autosomal Dominant Polycystic Kidney Disease. Am J Physiol Ren. Physiol.   288, (2005). human ICC-IF
Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417–430 (2005). human WB
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003). human WB, ICC-IF
Langbein, L. et al. Tight junction-related structures in the absence of a lumen: Occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur. J. Cell Biol. 82, 385–400 (2003). bovine IHC (frozen)
Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–347 (2003). human WB, IHC (frozen)
Langbein, L. et al. Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom. Eur. J. Cell Biol. 81, 419–435 (2002). bovine IHC (frozen)
Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209–222 (2000). human ICC-IF
Hofmann, I. et al. Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis. J. Cell Sci. 113, 2471–83 (2000). human IP
Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359–369 (1996). human ICC-IF
Owaribe, K. et al. The hemidesmosomal plaque. I. Characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation. 45, 207–20 (1990). bovine IHC (frozen)
Cowln, P., Kapprell, H.-P. & Franke, W. W. The Complement of Desmosomal Plaque Proteins in Different Cell Types. J. Cell Biol. 101, 1442–1454 (1985). bovine WB, IHC (frozen)

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