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anti-Desmoplakin 1/2 mouse monoclonal, DP I/II 236.23.1, supernatant

The anti-desmoplakin 1/2 antibody decorates desmoplakin 1 and 2 in desmosomal complexes. The anti-desmoplakin 1/2 antibody is useful for staining of primary and metastatic carcinoma.
Cat. No.: 651109
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone DP I/II 236.23.1
Immunogen C-terminal polypeptide of recombinant human desmoplakin (starting at amino acid position 1980)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, Xenopus laevis

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The antibody shows distinct punctate membrane staining of different epithelia. Desmosomes of the myocardium are also positive. Tumors specifically detected: Primary and metastatic carcinoma and meningioma. Polypeptides reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000)

Reactivity on cultured cell lines: Several human carcinoma cell lines: MCF-7,A-431, HaCaT, CaCo2; bovine cells: MDBK, BMGE

Reference

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