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anti-Desmoplakin 1/2 mouse monoclonal, DP447, supernatant

The anti-desmoplakin 1/2 antibody decorates desmoplakin 1 and 2 in desmosomal complexes. The anti-desmoplakin 1/2 antibody is useful for staining of primary and metastatic carcinoma.
Cat. No.: 651155
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DP447
Immunogen synthetic peptide of human desmoplakin 2 (aa 2115-2132)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, human, mouse, trout, Xenopus laevis, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The antibody shows distinct punctate membrane staining of different epithelia. Area compositae of the myocardium of higher vertebrates are also positive. The epitope is located in a region highly conserved among species (human, zebrafish).
Polypeptides reacting: Desmoplakin 1 and 2.

Reactivity on cultured cell lines: Several human carcinoma cell lines: MCF-7, A-431, HaCaT, CaCo2; bovine cells: MDBK, BMGE; RTG-2 (rainbow trout).

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