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anti-Desmoplakin 1 guinea pig polyclonal, serum

The anti-desmoplakin 1 antibody reacts specifically with desmoplakin 1 in Western blot analysis.
Cat. No.: DP-1
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€238.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen human desmoplakin 1 (C-terminus of recombinant human desmoplakin 1)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100
Immunohistochemistry (IHC) - frozen 1:50
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended, ABC method)
Western Blot (WB) 1:1,000

Background

Details

DP-1 antiserum shows distinct punctate membrane staining of epithelia. In Western blot analysis the antiserum reacts specifically with the desmoplakin DPI and DPII polypeptides.
Reactivity on cultured cell lines: Several human carcinoma cell lines, such as MCF-7, A-431, HaCaT, CaCo2

Reference

Reference

  1. König, K. et al. Loss of the keratin cytoskeleton is not sufficient to induce epithelial mesenchymal transition in a novel KRAS driven sporadic lung cancer mouse model. PLoS One 8, e57996 (2013).
  2. Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750–1758 (2012).
  3. Ibi, M. et al. Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. J. Cell Sci. 124, 857–864 (2011).
  4. Breuninger, S. et al. Desmosomal Plakophilins in the Prostate and Prostatic Adenocarcinomas Implications for Diagnosis and Tumor Progression. Am. J. Pathol. 176, 2509–2519 (2010).
  5. Silberberg, M., Charron, A. J., Bacallao, R. & Wandinger-Ness, A. Mispolarization of Desmosomal Proteins and Altered Cell Adhesion in Autosomal Dominant Polycystic Kidney Disease. Am J Physiol Ren. Physiol.   288, (2005).
  6. Miravet, S. et al. Tyrosine Phosphorylation of Plakoglobin Causes Contrary Effects on Its Association with Desmosomes and Adherens Junction Components and Modulates beta-Catenin-Mediated Transcription. Mol. Cell. Biol. 23, 7391–7402 (2003).
  7. Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–47 (2003).
  8. Weiske, J. et al. The Fate of Desmosomal Proteins in Apoptotic Cells. J. Biol. Chem. 276, 41175–41181 (2001).
  9. Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209–222 (2000).
  10. Due, W. et al. Testicular sex cord stromal tumour with granulosa cell differentiation: detection of steroid hormone receptors as a possible basis for tumour development and therapeutic management. J Clin Pathol 43, 732–737 (1990).

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