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anti-Desmoplakin 1 guinea pig polyclonal, serum Zoom

anti-Desmoplakin 1 guinea pig polyclonal, serum

The anti-desmoplakin 1 antibody reacts specifically with desmoplakin 1 in Western blot analysis.
Cat. No.: DP-1
Quantity:  50 µL
No. of experiments:  ≤ 500 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$298.00
Excl. VAT, excl. Shipping Cost

Filter

Publication
Species
Application
http://dx.plos.org/10.1371/journal.pone.0004194
human
IHC-IF
Ollech, D. et al. An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells. Nat.Commun. 11, 472 (2020)
human
ICC-IF
Moch, M. et al. The keratin-desmosome scaffold: pivotal role of desmosomes for keratin network morphogenesis. Cell.Mol.Life.Sci. , (2019)
human
ICC-IF
Duchatelet, S. et al. Mutations in PERP Cause Dominant and Recessive Keratoderma. J.Invest.Dermatol. 139, 380-390 (2019)
human
IHC-IF (paraffin)
Kant, S. et al. Desmoglein 2 mutation provokes skeletal muscle actin expression and accumulation at intercalated discs in murine hearts. J.Cell.Sci. 132, (2019)
mouse
IHC-IF (paraffin)
Boyden, L. et al. Recessive Mutations in AP1B1 Cause Ichthyosis, Deafness, and Photophobia. Am.J.Hum.Genet. 105, 1023-1029 (2019)
human
IHC-IF
Kant, S., Holthöfer, B., Magin, T. M., Krusche, C. A. & Leube, R. E. Desmoglein 2-Dependent Arrhythmogenic Cardiomyopathy Is Caused by a Loss of Adhesive Function. Circ. Cardiovasc. Genet. 8, 553–63 (2015).
mouse
IHC (paraffin)
Franke, W. W. et al. Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology. Cell Tissue Res. 357, 159–72 (2014
rat
König, K. et al. Loss of the keratin cytoskeleton is not sufficient to induce epithelial mesenchymal transition in a novel KRAS driven sporadic lung cancer mouse model. PLoS One 8, e57996 (2013).
human
ICC-IF
Langbein, L. et al. Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur. J. Cell Biol. 82, 385–400 (
bovine
Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–347 (2003).
human
Franke, W. W. et al. Immunocytochemical identification of epithelium-derived human tumors with antibodies to desmosomal plaque proteins (cell junctions/cytoskeleton/tumor diagnosis/carcinoma). Proc. Natl. Acad. Sci. USA 80, 543–547 (1983).
human
WB
ICC-IF

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen human desmoplakin 1 (C-terminus of recombinant human desmoplakin 1)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 500 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100
Immunohistochemistry (IHC) - frozen 1:50
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended, ABC method)
Western Blot (WB) 1:1,000

Background

Details

DP-1 antiserum shows distinct punctate membrane staining of epithelia. In Western blot analysis the antiserum reacts specifically with the desmoplakin DPI and DPII polypeptides.
Reactivity on cultured cell lines: Several human carcinoma cell lines, such as MCF-7, A-431, HaCaT, CaCo2

Reference

Filter

Publication
Species
Application
http://dx.plos.org/10.1371/journal.pone.0004194
human
IHC-IF
Ollech, D. et al. An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells. Nat.Commun. 11, 472 (2020)
human
ICC-IF
Moch, M. et al. The keratin-desmosome scaffold: pivotal role of desmosomes for keratin network morphogenesis. Cell.Mol.Life.Sci. , (2019)
human
ICC-IF
Duchatelet, S. et al. Mutations in PERP Cause Dominant and Recessive Keratoderma. J.Invest.Dermatol. 139, 380-390 (2019)
human
IHC-IF (paraffin)
Kant, S. et al. Desmoglein 2 mutation provokes skeletal muscle actin expression and accumulation at intercalated discs in murine hearts. J.Cell.Sci. 132, (2019)
mouse
IHC-IF (paraffin)
Boyden, L. et al. Recessive Mutations in AP1B1 Cause Ichthyosis, Deafness, and Photophobia. Am.J.Hum.Genet. 105, 1023-1029 (2019)
human
IHC-IF
Kant, S., Holthöfer, B., Magin, T. M., Krusche, C. A. & Leube, R. E. Desmoglein 2-Dependent Arrhythmogenic Cardiomyopathy Is Caused by a Loss of Adhesive Function. Circ. Cardiovasc. Genet. 8, 553–63 (2015).
mouse
IHC (paraffin)
Franke, W. W. et al. Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology. Cell Tissue Res. 357, 159–72 (2014
rat
König, K. et al. Loss of the keratin cytoskeleton is not sufficient to induce epithelial mesenchymal transition in a novel KRAS driven sporadic lung cancer mouse model. PLoS One 8, e57996 (2013).
human
ICC-IF
Langbein, L. et al. Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur. J. Cell Biol. 82, 385–400 (
bovine
Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–347 (2003).
human
Franke, W. W. et al. Immunocytochemical identification of epithelium-derived human tumors with antibodies to desmosomal plaque proteins (cell junctions/cytoskeleton/tumor diagnosis/carcinoma). Proc. Natl. Acad. Sci. USA 80, 543–547 (1983).
human
WB
ICC-IF

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