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anti-Desmoplakin 1 guinea pig polyclonal, serum

The anti-desmoplakin 1 antibody reacts specifically with desmoplakin 1 in Western blot analysis.
Cat. No.: DP-1
Quantity:  50 µL

Delivery Time: usually 1-7 working days

$298.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen human desmoplakin 1 (C-terminus of recombinant human desmoplakin 1)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100
Immunohistochemistry (IHC) - frozen 1:50
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended, ABC method)
Western Blot (WB) 1:1,000

Background

Details

DP-1 antiserum shows distinct punctate membrane staining of epithelia. In Western blot analysis the antiserum reacts specifically with the desmoplakin DPI and DPII polypeptides.
Reactivity on cultured cell lines: Several human carcinoma cell lines, such as MCF-7, A-431, HaCaT, CaCo2

Reference

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