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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | human desmoplakin 1 (C-terminus of recombinant human desmoplakin 1) |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | bovine, human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:100 |
| Immunohistochemistry (IHC) - frozen | 1:50 |
| Immunohistochemistry (IHC) - paraffin | 1:50 (microwave treatment recommended, ABC method) |
| Western Blot (WB) | 1:1,000 |
Background
Details
DP-1 antiserum shows distinct punctate membrane staining of epithelia. In Western blot analysis the antiserum reacts specifically with the desmoplakin DPI and DPII polypeptides.
Reactivity on cultured cell lines: Several human carcinoma cell lines, such as MCF-7, A-431, HaCaT, CaCo2
Reference
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