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anti-Desmoplakin 1 mouse monoclonal, DP-2.17, lyophilized, purified

The monoclonal anti-desmoplakin 1 antibody recognizes desmoplakin 1 of desmosomal complexes. The anti-desmoplakin 1 antibody is useful for identification of primary and metastatic carcinoma.
Cat. No.: 61024
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€201.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DP2.17
Immunogen bovine desmoplakin 1
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

DP2.17 shows distinct punctate membrane staining of epithelia. DP2.17 reacts with the rod fragment of desmoplakin 1.
Polypeptide reacting: Desmoplakin no. 1 (Mr 250,000 polypeptide of desmosomal plaques)
Reactivity on cultured cell lines: Several human carcinoma cell lines, such as MCF-7, A-431, TR-146; bovine cells: MDBK, BMGE
Tumors specifically detected: Primary and metastatic carcinoma; meningioma

Reference

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