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IHC of human colon using anti-Plakophilin 2 antibody (Cat. No. 651101) (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human colon using anti-Plakophilin 2 antibody (Cat. No. 651101) (courtesy of J.Heß, University Hospital Heidelberg)

anti-Desmosome sample set 2

The anti-desmosome sample set 2 provides antibodies directed against Desmoglein 1/2, Plakophilin 2, Plakoglobin and Desmoplakin 1/2 to evaluate their presence and status in IHC and WB.  The set contains enough antibody to perform stainings on 6-12 sections per antibody.

highly_published external_validation
Cat. No.: 70031
Quantity:  600 µl each antibody

 

Cat. No. Product Name Reactivity Application
61002S anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, purified, sample (ready-to-use) bovine, human, rat ICC/IF, IHC, WB
651101S anti-Plakophilin 2 mouse monoclonal, PP2/62 + PP2/86 + PP2/150, supernatant, sample (ready-to-use) bovine, human, rat IHC, WB
61005S anti-Plakoglobin mouse monoclonal, PG 5.1, purified, sample (ready-to-use) bovine, chicken, human, mouse, rat, zebrafish ICC/IF, IHC, WB
61003S anti-Desmoplakin 1/2 mouse monoclonal, DP 1 + 2-2.15, purified, sample (ready-to-use) bovine, chicken, human, mouse, rat ICC/IF, IHC, WB

Price as configured: $184.00

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Number of experiments 6-12 sections per antibody
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

As one of the stronger cell-to-cell adhesion types desmosomes allow cells to connect their intemediate filaments to those of neighboring cells. Therefore desmosomes can be found especially in cardiac muscle tissue, in bladder tissue and in epithelia, where the meachnical stress is quite high. As members of the cadherin super family, desmogleins and desmocollins thereby mediate adhesion at demosomes. Plakoglobin and the plakophilins on the other hand are members of the armadillo gene family and mediate the connection of the desmosome to desmoplakins that themselves mediate the connection to the cytoskeleton.

Mutations in these proteins or autoimmune reaction against them lead to severe diseases like arrhythmogenic right ventricular cardiomyopathy, Hailey-Hailey disease or striate palmoplantar keratoderma.

Furthermore the tissue-specific expression of desmosomal proteins is critical to tissue homeostasis. Missexpression of single components of desmosomes can lead to hyperproliferation and abnormal differentiation.

The anti-desmosome sample set 2 provides antibodies directed against Desmoglein 1/2, Plakophilin 2, Plakoglobin and Desmoplakin 1/2 to evaluate their presence and status in IHC and WB.  The set contains enough antibody to perform stainings on 6-12 sections per antibody.

Set content:
Cat. No. 61002S, anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, purified, sample (ready-to-use), 600 µl
Cat. No. 651101S, anti-Plakophilin 2 mouse monoclonal, PP2/62 + PP2/86 + PP2/150, supernatant, sample (ready-to-use), 600 µl
Cat. No. 61005S, anti-Plakoglobin mouse monoclonal, PG 5.1, purified, sample (ready-to-use), 600 µl
Cat. No. 61003S, anti-Desmoplakin 1/2 mouse monoclonal, DP 1 + 2-2.15, purified, sample (ready-to-use), 600 µl

Reference

Reference

PublicationSpeciesApplicationAntibody
Shafraz, O. et al. E-cadherin binds to desmoglein to facilitate desmosome assembly. Elife. 7,  (2018). mouse ICC-IF 61003
Niell, N. et al. The human PKP2/plakophilin-2 gene is induced by Wnt/β-catenin in normal and colon cancer-associated fibroblasts. Int.J.Cancer. 142, 792-804 (2018). human WB 651101
Price, A. et al. Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Nat.Commun. 9, 5284 (2018). mouse ICC-IF 61002
Dubash, A. D. et al. Plakophilin-2 loss promotes TGF-β1/p38 MAPK-dependent fibrotic gene expression in cardiomyocytes. J. Cell Biol. 212, 425–38 (2016). rat WB, ICC-IF 651101
Kant, S., Holthöfer, B., Magin, T. M., Krusche, C. A. & Leube, R. E. Desmoglein 2-Dependent Arrhythmogenic Cardiomyopathy Is Caused by a Loss of Adhesive Function. Circ. Cardiovasc. Genet. 8, 553–63 (2015). mouse IHC (paraffin) 651101
Izawa, G., Kobayashi, W., Haraguchi, M., Sudo, A. & Ozawa, M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int. J. Mol. Med. 36, 166–72 (2015). bovine WB 61002
Noritake, K., Aki, T., Funakoshi, T., Unuma, K. & Uemura, K. Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes. PLoS One (2015). mouse WB 61002
Rappe, U., Schlechter, T., Aschoff, M., Hotz-Wagenblatt, A. & Hofmann, I. Nuclear ARVCF Protein Binds Splicing Factors and Contributes to the Regulation of Alternative Splicing. J. Biol. Chem. 289, 12421–12434 (2014). human WB 651101
Patel, D. M., Dubash, A. D., Kreitzer, G. & Green, K. J. Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin-EB1 interactions. J. Cell Biol. 206, 779–97 (2014). human ICC-IF 651101
Fischer-Kešo, R. et al. Plakophilins 1 and 3 Bind to FXR1 and Thereby Influence the mRNA Stability of Desmosomal Proteins. Mol. Cell. Biol. 34, 4244–4256 (2014). human WB, IP 651101
Dayal, J. H. S. et al. Type VII collagen regulates expression of OATP1B3, promotes front-to-rear polarity and increases structural organisation in 3D spheroid cultures of RDEB tumour keratinocytes. J. Cell Sci. 127, 740–51 (2014). human ICC-IF 61005
Jennemann, R. et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum. Mol. Genet. 21, 586–608 (2012). mouse WB 61005, 61002
Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750–1758 (2012). mouse IHC (frozen) 61003
Rickelt, S. Plakophilin-2: a cell-cell adhesion plaque molecule of selective and fundamental importance in cardiac functions and tumor cell growth. Cell Tissue Res 348, 281–294 (2012). human, mouse WB, IHC (paraffin) 651101
Pieperhoff, S. et al. The plaque protein myozap identified as a novel major component of adhering junctions in endothelia of the blood and the lymph vascular systems. J. Cell. Mol. Med 16, 1709–1719 (2012). mouse WB 651101
Fabritz, L. et al. Load-reducing therapy prevents development of arrhythmogenic right ventricular cardiomyopathy in plakoglobin-deficient mice. J. Am. Coll. Cardiol. 57, 740–750 (2011). mouse IHC 651101
Fabritz, L. et al. Load-Reducing Therapy Prevents Development of Arrhythmogenic Right Ventricular Cardiomyopathy in Plakoglobin-Deficient Mice. J. Am. Coll. Cardiol. 57, 740–750 (2011). mouse WB, IHC 61005
Lombardi, R. et al. Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy. Circ. Res. 109, 1342–53 (2011). mouse Co-IP 61002
Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells. Int. J. cancer 125, 2036–48 (2009). human WB, ICC-IF

651101, 61002

Goossens, S. et al. A unique and specific interaction between  T-catenin and plakophilin-2 in the area composita, the mixed-type junctional structure of cardiac intercalated discs. J. Cell Sci. 120, 2126–2136 (2007). mouse WB, IHC (paraffin), IEM 651101
Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates – IV: Coalescence and amalgamation of desmosomal and adhaerens junction components – Late processes in mammalian heart development. Eur. J. Cell Biol. 86, 377–391 (2007). mouse IHC (frozen) 651101, 61002
Boehnke, K. et al. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur. J. Cell Biol. 86, 731–746 (2007). human IHC (paraffin) 61005
Borrmann, C. M. et al. The area composita of adhering junctions connecting heart muscle cells of vertebrates. II. Colocalizations of desmosomal and fascia adhaerens molecules in the intercalated disk. Eur. J. Cell Biol. 85, 469–485 (2006). human, mouse, rat, bovine, eel, carp WB, IHC (frozen)

61005, 61002

Franke, W. W., Borrmann, C. M., Grund, C. & Pieperhoff, S. The area composita of adhering junctions connecting heart muscle cells of vertebrates. I. Molecular definition in intercalated disks of cardiomyocytes by immunoelectron microscopy of desmosomal proteins. European Journal of Cell Biology 85, 69–82 (2006). human IEM

61002

Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417–430 (2005). human WB, IP 61005
Biedermann, K. et al. Desmoglein 2 is expressed abnormally rather than mutated in familial and sporadic gastric cancer. J. Pathol. 207, 199–206 (2005). human IHC (paraffin) 61059
Bosch, F. X., Andl, C., Abel, U. & Kartenbeck, J. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: A comparison of E-cadherin with desmosomal components. Int. J. Cancer 114, 779–790 (2005). human IHC (frozen) 61003, 61002
Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761–774 (2005). human IHC (frozen), ICC-IF 61005, 61002
Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417–30 (2005). human IHC (paraffin), IEM 61002
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003). human WB, ICC-IF 61005, 61002
Miravet, S. et al. Tyrosine Phosphorylation of Plakoglobin Causes Contrary Effects on Its Association with Desmosomes and Adherens Junction Components and Modulates ␤-Catenin-Mediated Transcription. Mol. Cell. Biol. 23, 7391–7402 (2003). human WB 61003
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003). human WB, ICC-IF 651101
South, A. P. et al. Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. J. Cell Sci. 116, 3303–3314 (2003). human WB 651101
Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–347 (2003). human WB, IHC-IF 651101
Weiske, J. et al. The Fate of Desmosomal Proteins in Apoptotic Cells. J. Biol. Chem. 276, 41175–41181 (2001). human WB, ICC-IF 61003
Caldelari, R. et al. A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris. J. Cell Biol. 153, 823–834 (2001). mouse WB, ICC-IF 61005, 61002
Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209–222 (2000). human ICC-IF 61003, 61002
Moll, I., Houdek, P., Schäfer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437–46 (1999). human IHC (frozen) 61003
Mertens, C., Kuhn, C., Moll, R., Schwetlick, I. & Franke, W. W. Desmosomal plakophilin 2 as a differentiation marker in normal and malignant tissues. Differentiation 64, 277–290 (1999). human, bovine IHC (frozen), ICC-IF 651101
Molj, I., Kurzcn, H., Langbein, L., Erner, W. & Franket, W. The Distribution of the DesmosoInal Protein, Plakophilin 1, in Human Skin and Skin Tumors. J. Invest. Dermatol. 108, 139–146 (1997). human IHC (frozen) 61003
Molj, I., Kurzcn, H., Langbein, L., Erner, W. & Franket, W. The Distribution of the DesmosoInal Protein, Plakophilin 1, in Human Skin and Skin Tumors. J. Invest. Dermatol. 108, 139–146 (1997). human IHC (frozen) 61005
Mertens, C., Kulm, C. & Franke, W. W. Plakophilins 2a and 2b: Constitutive Proteins of Dual Location in the Karyoplasm and the Desmosomal Plaque. J. Cell Biol. 135, 1009–1025 (1996). human, bovine IHC (frozen), ICC-IF 61005
Mertens, C., Kulm, C. & Franke, W. W. Plakophilins 2a and 2b: Constitutive Proteins of Dual Location in the Karyoplasm and the Desmosomal Plaque. J. Cell Biol. 135, 1009–1025 (1996). human, bovine IHC (frozen), ICC-IF 651101
Wahl, J. K. et al. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J. Cell Sci. 109, 1143–54 (1996). human WB 61005
Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359–369 (1996). human WB, ICC-IF

61005, 61002

Schäfer, S., Stumpp, S. & Franke, W. W. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 60, 99–108 (1996). human WB 61002
Leube, R. E. The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108, 883–894 (1995). human ICC-IF 61002
Schmelz, M. & Franke, W. W. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274–89 (1993). human, rat, bovine WB, IHC (frozen), ICC-IF, IEM 61002
Owaribe, K., Nishizawa, Y. & Franke, W. W. Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Res. 192, 622–30 (1991). bovine IHC (frozen) 61003
Owaribe, K. et al. The hemidesmosomal plaque. I. Characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation. 45, 207–20 (1990). rat, bovine IHC (frozen) 61003
Rungger-Brandle, E., Achtstatter, T. & Franke, W. W. An epithelium-type cytoskeleton in a glial cell: Astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes. J. Cell Biol. 109, 705–716 (1989). R. ridibunda IHC (frozen) 61003
Achtstätter, T., Fouquet, B., Rungger‐BraUndele, E. & Frnake, W. W. Cytokeratin filaments and desmonsomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species. Differentiation 40, 129–149 (1989). human IHC (frozen) 61005
Moll, I; Heid, H; Moll, R. Cytokeratin analysis of pilomatrixoma: changes in cytokeratin-type expression during differentiation. J. Invest. Dermatol. 91, 251–7 (1988). human IHC (frozen) 61003
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987). human IHC (frozen) 61003
Dockhorn-Dworniczak, B. et al. Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. Differentiation. 35, 53–71 (1987). human IHC (frozen) 61003
Achtstätter, T. et al. Expression of glial filament protein (GFP) in nerve sheaths and non-neural cells re-examined using monoclonal antibodies, with special emphasis on the co-expression of GFP and cytokeratins in epithelial cells of human salivary gland and pleomorphic. Differentiation 31, 206–227 (1986). human IHC (frozen) 61003
Cowin, P., Kapprell, H. P., Franke, W. W., Tamkun, J. & Hynes, R. O. Plakoglobin: a protein common to different kinds of intercellular adhering junctions. Cell 46, 1063–73 (1986). human, bovine WB, ICC-IF, IEM 61005
Cowln, P., Kapprell, H.-P. & Franke, W. W. The Complement of Desmosomal Plaque Proteins in Different Cell Types. J. Cell Biol. 101, 1442–1454 (1985) human, bovine IHC (frozen), IEM 61003

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