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anti-DNA mouse monoclonal, AC-30-10, lyophilized, purified

The anti-DNA antibody reacts with all forms of native and denatured DNA. Useful for the identification of mycoplasma contamination.
Cat. No.: 61014
Quantity:  100 µg
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$281.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone AC-30-10
Immunogen double- and single-stranded DNA
Purification size exclusion chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity all species

Applications

Tested applications Tested dilutions
Dot Blot assay dependent (on nitrocellulose membrane, after baking at 70°C)
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)

Background

Details

AC-30-10 stains DNA present in the nuclei and the mitochondria of cells and tissues. It is also a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. Due to the high sensitivity it can also be used for the detection of mycoplasma contamination in cell cultures.
Binding characteristics: Reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA
Tested cultured cell lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa

Reference

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