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anti-Enkephalin rabbit polyclonal, serum

Enkephalins are the most abundant opioid peptides in the body. Several types of neuroendocrine tumors, including pheochromocytomas, neuroblastomas and bronchial and gastroinestinal endocrine tumors are stained positively.
Cat. No.: 16020
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€245.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host rabbit
Antibody Type polyclonal
Immunogen synthetic met-enkephalin (Sigma), conjugated to BSA
Purification undiluted antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 100 µl dist. water
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity cat, pig, rat
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:400 - 1:800
Immunohistochemistry (IHC) - paraffin 1:400 - 1:800 (microwave treatment recommended)

Background

Details

Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, incl. pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.
Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while ß-endorphin does not. Cross-reacts with leu-enkephalin.

Positive control: Frozen sections of cat or pig small intestine.

Reference

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