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Product description
| Host | rabbit |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic met-enkephalin (Sigma), conjugated to BSA |
| Purification | undiluted antiserum |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 100 µl dist. water |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 1600 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | cat, pig, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:400 - 1:800 |
| Immunohistochemistry (IHC) - paraffin | 1:400 - 1:800 (microwave treatment recommended) |
Background
Details
Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, incl. pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.
Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while ß-endorphin does not. Cross-reacts with leu-enkephalin.
Positive control: Frozen sections of cat or pig small intestine.
Reference
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Species
Application
rat
guinea pig
guinea pig
whole mount
rat
IHC (free floating sections)
