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human head and neck squamous-cell carcinoma (HNSCC)(courtesy of J.Heß, University Hospital Heidelberg) Zoom

human head and neck squamous-cell carcinoma (HNSCC)(courtesy of J.Heß, University Hospital Heidelberg)

anti-EP-CAM mouse monoclonal, HEA125, lyophilized, purified

The antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34) widely expressed on epithelia and tumors derived therefrom. Excellent marker to discriminate epithelial from mesothelial structures.
application_confirmed highly_published external_validation
Cat. No.: 61004
Quantity:  50 µg
No. of experiments:  ≤ 1000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$289.00
Excl. VAT, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone HEA125
Immunogen HT-29 colon carcinoma cell line
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID P16422
No. of experiments ≤ 1000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity human (negative with mouse)

Applications

Tested applications Tested dilutions
Flow Cytometry (FACS) assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10 - 1:50 (1-5 µg/mL)
Immunohistochemistry (IHC) - paraffin 1:10 - 1:50 (1-5 µg/mL; protease treatment recommended)
Western Blot (WB) assay dependent (non-reducing conditions)

Background

Details

The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen, EPG34, CD326, and TACSTD1). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative.

The antibody can be used as alternative to the anti-EpCAM antibody clone BerEp4.

Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).

Reactivity on cultured cell lines: All carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines.

Reference

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