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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | VU-1D9 |
| Immunogen | isolated from small cell lung carcinoma-derived cell line (NC1-H69) |
| Purification | ascites |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10 - 1:20 |
| Immunohistochemistry (IHC) - paraffin | 1:10 - 1:20 (microwave treatment recommended) |
Background
Details
Ep-CAM (homophylic cell-cell adhesion molecule, also named ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a 40 kD epithelial protein expressed on the baso-lateral cell surface of many epithelial tissues. It is a transmembrane glycoprotein with 3 potential glycosyla-tion sites. The extracellular domain has a cysteine-rich repeat and a small domain with homology to nidogen. Antibody stains most epithelial cells and carcinoma de-rived therefrom.
Positive control: Breast carcinoma.
Reference
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