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anti-EP-CAM mouse monoclonal, VU-1D9, ascites fluid

Ep-CAM (homophylic cell-cell adhesion molecule, also named ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a 40 kD epithelial protein expressed on the baso-lateral cell surface of many epithelial tissues. Antibody stains most epithelial cells and carcinoma de-rived therefrom.
Cat. No.: 16114
Quantity:  1 mL
No. of experiments:  ≤ 400 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$326.00
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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone VU-1D9
Immunogen isolated from small cell lung carcinoma-derived cell line (NC1-H69)
Purification ascites
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10 - 1:20
Immunohistochemistry (IHC) - paraffin 1:10 - 1:20 (microwave treatment recommended)

Background

Details

Ep-CAM (homophylic cell-cell adhesion molecule, also named ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a 40 kD epithelial protein expressed on the baso-lateral cell surface of many epithelial tissues. It is a transmembrane glycoprotein with 3 potential glycosyla-tion sites. The extracellular domain has a cysteine-rich repeat and a small domain with homology to nidogen. Antibody stains most epithelial cells and carcinoma de-rived therefrom.

Positive control: Breast carcinoma.

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