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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic human gastrin I, conjugated to BSA |
| Purification | undiluted antiserum |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 100 µl dist. water |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 3000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:1,000 - 1:1,500 |
| Immunohistochemistry (IHC) - frozen | 1:1,000 - 1:1,500 |
| Immunohistochemistry (IHC) - paraffin | 1:1,000 - 1:1,500 (microwave treatment recommended) |
Background
Details
Gastrin-secreting cells are numerous in the antrum and a few are found in the proximal duodenum. The antibody can be used for the diagnosis of gastrin-producing tumors which are mainly found in the pancreas and occasionally in the stomach and the duodenum.
Absorption with 10 – 100 µg gastrin 1-34 and CCK 8 per ml antiserum abolishes the staining.
Positive control: Formalin-fixed paraffin sections of rat antrum.
Reference
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