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| Cat. No. | Product Name | Format | Quantity | Price |
|---|
Product description
| Host | goat |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | guinea pig IgG |
| Purification | affinity chromatography |
| Conjugate | peroxidase/ HRP |
| Formulation | lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 20000 (WB, 5 ml reaction volume) |
| Intended use | Research use only |
| Tested species reactivity | guinea pig |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | 1:1,250 - 1:25,000 |
| Western Blot (WB) | 1:2,500 - 1:100,000 |
Background
Details
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
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