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anti-Hantavirus Puumala mouse monoclonal, A1C5, purified

The antibody is directed against the Puumala serotype nucleocapsid protein which has been shown to be the major immunogen in infections (HFRS, Nephropathia epidemica) caused by Puumala Virus.
Positive cross-reaction with Sin Nombre Virus (SNV).
No coss-reaction with Hantaan-related serogroups, like Dobrava, Seoul.
Cat. No.: A1C5-P
Quantity:  100 µg

Delivery Time: usually 1-7 working days

$245.00
Excl. VAT, excl. Shipping Cost

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Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
A1C5-C

anti-Hantavirus Puumala mouse monoclonal, A1C5

supernatant 400 µL
$141.00
Excl. VAT, excl. Shipping Cost
A1C5-POD

anti-Hantavirus Puumala POD mouse monoclonal, A1C5

Peroxidase Conjugate 200 µL
$259.00
Excl. VAT, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone A1C5
Immunogen recombinant Puumala nucleocapsid protein
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity Puumala virus-infected cells

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50
Immunohistochemistry (IHC) - frozen assay dependent
Western Blot (WB) assay dependent

Background

Details

A1C5 reacts with an epitope present on the N-terminus (between aa 15 and 35) of the nucleocapsid protein (S segment) of hantavirus CG 18-20 strain (Puumala serotype) and also cross-reacts with SNV (Sin Nombre virus) rN protein (Schmidt et al. 2005).

Schmidt, J., Meisel, H., Hjelle, B., Krüger, D. H. & Ulrich, R. Development and evaluation of serological assays for detection of human hantavirus infections caused by Sin Nombre virus. J. Clin. Virol. Off. Publ. Pan Am. Soc. Clin. Virol. 33, 247–53 (2005).

Reference

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