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anti-Hantavirus Puumala POD mouse monoclonal, A1C5, Peroxidase Conjugate

The antibody is directed against the Puumala serotype nucleocapsid protein which has been shown to be the major immunogen in infections (HFRS, Nephropathia epidemica) caused by Puumala Virus.
Cat. No.: A1C5-POD
Quantity:  200 µL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$259.00
Excl. VAT, excl. Shipping Cost

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Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
A1C5-C

anti-Hantavirus Puumala mouse monoclonal, A1C5

supernatant 400 µL
$141.00
Excl. VAT, excl. Shipping Cost
A1C5-P

anti-Hantavirus Puumala mouse monoclonal, A1C5

purified 100 µg
$245.00
Excl. VAT, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone A1C5
Immunogen recombinant Puumala nucleocapsid protein
Purification protein A affinity chromatography
Conjugate peroxidase/ HRP
Formulation PBS with BSA and 0.001% thimerosal
Storage store at 2-8°C
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity Puumala virus-infected cells

Applications

Tested applications Tested dilutions
ELISA 1:50
Immunohistochemistry (IHC) - frozen 1:50
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The antibody is directed against the Puumala serotype nucleocapsid protein which has been shown to be the major immunogen in infections (HFRS, Nephropathia epidemica) caused by Puumala Virus.
A1C5 reacts with an epitope present on the N-terminus (aa 15 - 35) of the nucleocapsid protein (S segment) of hantavirus CG 18-20 strain (Puumala serotype) and also cross-reacts with SNV (Sin Nombre virus) rN protein (Schmidt et al. 2005).
No coss-reaction with Hantaan-related serogroups, like Dobrava, Seoul.

Schmidt, J., Meisel, H., Hjelle, B., Krüger, D. H. & Ulrich, R. Development and evaluation of serological assays for detection of human hantavirus infections caused by Sin Nombre virus. J. Clin. Virol. Off. Publ. Pan Am. Soc. Clin. Virol. 33, 247–53 (2005).

Reference

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