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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| A1C5-C |
anti-Hantavirus Puumala mouse monoclonal, A1C5 |
supernatant | 400 µL |
$141.00
Excl. VAT, excl. Shipping Cost
|
| A1C5-P |
anti-Hantavirus Puumala mouse monoclonal, A1C5 |
purified | 100 µg |
$245.00
Excl. VAT, excl. Shipping Cost
|
Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | A1C5 |
| Immunogen | recombinant Puumala nucleocapsid protein |
| Purification | protein A affinity chromatography |
| Conjugate | peroxidase/ HRP |
| Formulation | PBS with BSA and 0.001% thimerosal |
| Storage | store at 2-8°C |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | Puumala virus-infected cells |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | 1:50 |
| Immunohistochemistry (IHC) - frozen | 1:50 |
| Immunohistochemistry (IHC) - paraffin | 1:50 (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
The antibody is directed against the Puumala serotype nucleocapsid protein which has been shown to be the major immunogen in infections (HFRS, Nephropathia epidemica) caused by Puumala Virus.
A1C5 reacts with an epitope present on the N-terminus (aa 15 - 35) of the nucleocapsid protein (S segment) of hantavirus CG 18-20 strain (Puumala serotype) and also cross-reacts with SNV (Sin Nombre virus) rN protein (Schmidt et al. 2005).
No coss-reaction with Hantaan-related serogroups, like Dobrava, Seoul.
Schmidt, J., Meisel, H., Hjelle, B., Krüger, D. H. & Ulrich, R. Development and evaluation of serological assays for detection of human hantavirus infections caused by Sin Nombre virus. J. Clin. Virol. Off. Publ. Pan Am. Soc. Clin. Virol. 33, 247–53 (2005).
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