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anti-Histamine rabbit polyclonal, serum

Histamine is a neurotransmitter in the central nervous system and mast cell constituent. In addition, histamine is produced in the endocrine cells (ECL-cells) in the oxyntic mucosa of the stomach.
Cat. No.: 16043
Quantity:  50 µL
No. of experiments:  ≤ 4000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$514.00
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Specifications

Product description

Host rabbit
Antibody Type polyclonal
Immunogen Histamine conjugated to HSA
Purification undiluted antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 100 µl dist. water
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 4000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity human, rat (most species)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:300-1:600
Immunohistochemistry (IHC) - frozen 1:1,500 – 1:2,000
Immunohistochemistry (IHC) - paraffin 1:1,500 – 1:2,000 (microwave treatment recommended)

Background

Details

Histamine is a neurotransmitter in the central nervous system as well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach. Absorption with 10-100 μg histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and histidine do not.

Positive control: Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach

Reference

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