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anti-Histidine Decarboxylase rabbit polyclonal, serum

Histidine decarboxylase catalyses the conversion of histidine into histamine. HDC can be found in the histamine secreting ECL cells of some species as well as in mast cells. The anti-Histidine Decarboxylase antibody is raised against recombinant rat HDC.
Cat. No.: 16045
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€411.00
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Product description

Host rabbit
Antibody Type polyclonal
Immunogen recombinant histidine decarboxylase produced in E. coli
Purification stabilized antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 50 - 100 μl dist. water (final solution contains 0.09 % sodium azide, 1% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity dog, guinea pig, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:1,000 – 1:1,500
Immunohistochemistry (IHC) - paraffin 1:1,000 – 1:1,500 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Histidine decarboxylase (HDC) is the enzyme catalyzing the conversion of histidine into histamine. HDC can be found in the histamine secreting ECL cells of some species as well as in the mast cells. Absorption with 10-100 μg immunogen per ml diluted antiserum abolishesthe staining.
In Western blot the antiserum detects the 54 kD and 73 kD forms in addition to a 63 kD form (rat stomach, see Dartsch et al., 1998).
Positive control: Stefanini-fixed frozen sections of rat fundus.


Dartsch, C., Chen, D. & Persson, L. Multiple forms of rat stomach histidine decarboxylase may reflect posttranslational activation of the enzyme. Regul. Pept. 77, 33–41 (1998).

Reference

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