anti-human IgE mouse monoclonal, MH25-1, purified
- Mouse monoclonal
- Suitable for ELISA, ICC/IF and IHC on frozen and paraffin sections
- Reacts with human
- Isotype: IgG2b
|Quantity||1 ml (200 µg/ml)|
|Application||ELISA, ICC/IF, IHC|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Isolated human immunoglobulins|
|Formulation||PBS with 0.5% BSA and 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:5-1:10|
|Immunohistochemistry (IHC) - paraffin||1:5-1:10 (proteolytic treatment with trypsin required)|
Mab MH25-1 reacts specifically with human IgE e-chains; De-1 epitope on Fc fragment. No cross reaction with other immunoglobulins.
Positive control: Polypus nose
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.