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Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG2b |
Clone | MH25-1 |
Immunogen | isolated human immunoglobulins |
Purification | affinity chromatography |
Conjugate | unconjugated |
Formulation | PBS with 0.5% BSA and 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | human |
Applications
Tested applications | Tested dilutions |
---|---|
ELISA | assay dependent |
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:5 - 1:10 |
Immunohistochemistry (IHC) - paraffin | 1:5 - 1:10 (proteolytic treatment with trypsin required) |
Background
Details
Mab MH25-1 reacts specifically with human IgE e-chains; De-1 epitope on Fc fragment. No cross reaction with other immunoglobulins.
Positive control: Polypus nose
Reference
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