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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG2b |
| Clone | MH25-1 |
| Immunogen | isolated human immunoglobulins |
| Purification | affinity chromatography |
| Conjugate | unconjugated |
| Formulation | PBS with 0.5% BSA and 0.09% sodium azide |
| Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | assay dependent |
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:5 - 1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:5 - 1:10 (proteolytic treatment with trypsin required) |
Background
Details
Mab MH25-1 reacts specifically with human IgE e-chains; De-1 epitope on Fc fragment. No cross reaction with other immunoglobulins.
Positive control: Polypus nose
