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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 kappa |
| Clone | MH16-1 |
| Immunogen | isolated human IgG |
| Purification | affinity chromatography |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human (no cross-reaction with goat, sheep, pig, horse, rabbit) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | assay dependent |
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:5 – 1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:5 – 1:10 |
Background
Details
MH16-1 recognizes IgG heavy chains. The epitope is present on the Fc part of IgG. No cross reactivity with IgA or IgM (tested with ELISA).
Suitable for lymphoma phenotyping. Multiple myelomas are of the IgG or IgA type.
Suitable for characterization of plasma cells in inflammatory conditions.
Positive control: tonsil.
Reference
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