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anti-Insulin/Proinsulin guinea pig polyclonal, serum

The antiserum was raised against human proinsulin. Absorption with 10 - 100 µg antigen inactivates the antiserum, while C-peptide and insulin only partly reduce staining.
Cat. No.: 16049
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€411.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic human proinsulin
Purification stabilized antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 50 - 100 μl dist. water (final solution contains 0.09 % sodium azide, 1% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity hamster, human, pig, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:1,000 – 1:2,000
Immunohistochemistry (IHC) - frozen 1:3,000 – 1:5,000 (PAP)
Immunohistochemistry (IHC) - paraffin 1:3,000 – 1:5,000 (PAP)

Background

Details

Insulin is produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.
Absorption with 10-100 μg proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and insulin only partly reduce staining.
Positive control: Formalin-fixed paraffin sections of human pancreas

Reference

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