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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic human proinsulin |
| Purification | undiluted antiserum |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 100 µl dist. water |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 10000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | hamster, human, pig, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:1,000 – 1:2,000 |
| Immunohistochemistry (IHC) - frozen | 1:3,000 – 1:5,000 (PAP) |
| Immunohistochemistry (IHC) - paraffin | 1:3,000 – 1:5,000 (PAP) |
Background
Details
Insulin is produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.
Absorption with 10-100 μg proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and insulin only partly reduce staining.
Positive control: Formalin-fixed paraffin sections of human pancreas
Reference
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Publication
Species
Application
rat
IHC
hamster
IHC (frozen)
