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Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | synthetic human proinsulin |
Purification | undiluted antiserum |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 100 µl dist. water |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 10000 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | hamster, human, pig, rat |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:1,000 – 1:2,000 |
Immunohistochemistry (IHC) - frozen | 1:3,000 – 1:5,000 (PAP) |
Immunohistochemistry (IHC) - paraffin | 1:3,000 – 1:5,000 (PAP) |
Background
Details
Insulin is produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.
Absorption with 10-100 μg proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and insulin only partly reduce staining.
Positive control: Formalin-fixed paraffin sections of human pancreas
Reference
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Publication
Species
Application
rat
IHC
hamster
IHC (frozen)