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human back skin (courtesy of L. Langbein) Zoom

human back skin (courtesy of L. Langbein)

anti-Keratin K1 guinea pig polyclonal, serum

The antibody is specific for keratin K1 in suprabasal layers of cornified squamous epithelia, e.g. the suprabasal layer of scalp and foot sole. Negative with non-keratinizing stratified and non-stratified epithelia (e.g. esophagus, duodenum).
Cat. No.: GP-K1
Quantity:  100 µL

Delivery Time: usually 1-7 working days

$311.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide (SAGIINYQRRTTSS - C) of human keratin K1 (formerly also designated cytokeratin 1), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) 1:3,000

Background

Details

Specific for keratin K1 in suprabasal layers of cornified squamous epithelia, e.g. the suprabasal layer of scalp and foot sole. Negative with non-keratinizing stratified and non-stratified epithelia (e.g. esophagus, duodenum)!
Polypeptide reacting: Basic human keratin K1 (Mr 66000)
Positive control: Human foot sole

Reference

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