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human scalp (courtesy of L. Langbein) Zoom

human scalp (courtesy of L. Langbein)

anti-Keratin K10 guinea pig polyclonal, serum

The anti-keratin k10 antibody is useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc.

application_confirmed external_validation
Cat. No.: GP-K10
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€262.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide of human keratin K10 (formerly also designated cytokeratin 10; C-GS VGE SSS KGP RY), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID P13645
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100 – 1:200
Immunohistochemistry (IHC) - paraffin 1:100 – 1:200 (microwave treatment recommended)
Western Blot (WB) 1:250 - 1:1,000

Background

Details

MW 56,5000 (pI 5.3) intermediate filament polypeptide, keratin K10, detected by immunohistochemistry in the suprabasal layers of human epidermis (basal cells are negative). Useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc. Completely negative on non-stratified epithelia.

Positive control: Human suprabasal keratinocytes of epidermis; squamous cell carcinoma

Reference

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