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| Cat. No. | Product Name | Format | Quantity | Price |
|---|
Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide of human keratin K10 (formerly also designated cytokeratin 10; C-GS VGE SSS KGP RY), coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| UniprotID | P13645 |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mouse, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:100 – 1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100 – 1:200 (microwave treatment recommended) |
| Western Blot (WB) | 1:250 - 1:1,000 |
Background
Details
MW 56,5000 (pI 5.3) intermediate filament polypeptide, keratin K10, detected by immunohistochemistry in the suprabasal layers of human epidermis (basal cells are negative). Useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc. Completely negative on non-stratified epithelia.
Positive control: Human suprabasal keratinocytes of epidermis; squamous cell carcinoma
Reference
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Species
Application
mouse
IHC-IF (paraffin)
mouse
IHC (paraffin)
