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anti-Keratin K10 mouse monoclonal, DE-K10, supernatant

MAb DE-K10 specifically recognizes the 56.6 kD keratin K10 polypeptide in squamous cell carcinomas from e.g. epidermis, lung, bladder, cervix, esophagus.
Cat. No.: 11414
Quantity:  1 mL

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DE-K10
Immunogen Cytoskeletal preparation from human epidermis
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human
Intended use Research use only


Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5 - 1:10
Immunohistochemistry (IHC) - paraffin 1:5 - 1:10 (protease treatment recommended)



MAb DE-K10 specifically recognizes the 56.6 kD keratin K10 (formerly also designated cytokeratin 10) polypeptide in squamous cells and carcinomas from e.g. epidermis, lung, bladder, cervix, oesophagus.
Positive control: Squamous cell carcinoma.



Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014). mouse IHC (frozen)
Langbein, L. et al. New facets of keratin K77: Interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793–812 (2013). human, mouse IHC (frozen)
Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750–1758 (2012). mouse IHC (frozen)
Boehnke, K. et al. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur. J. Cell Biol. 86, 731–46 (2007). human IHC (paraffin)
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Corver, W. E. et al. Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer. Cytometry 39, 96–107 (2000). human FACS
Moll, I., Houdek, P., Schäfer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437–46 (1999). human IHC (paraffin)

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