human skin (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K10 mouse monoclonal, DE-K10, supernatant

MAb DE-K10 specifically recognizes the 56.6 kD keratin K10 polypeptide in squamous cell carcinomas from e.g. epidermis, lung, bladder, cervix, esophagus.

Cat. No.: 11414
Quantity: 1 mL
No. of experiments: ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$285.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Boink, M. et al. Comparison of advanced therapy medicinal product gingiva and skin substitutes and their in vitro wound healing potentials. J.Tissue.Eng.Regen.Med. 12, e1088-e1097 (2018).

human

IHC (paraffin)

Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017).

human

IHC-IF (paraffin)

Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014).

mouse

IHC (frozen)

Langbein, L. et al. New facets of keratin K77: Interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793â812 (2013).

human
mouse

IHC (frozen)

Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750â1758 (2012).

mouse

IHC (frozen)

Boehnke, K. et al. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur. J. Cell Biol. 86, 731â746 (2007).

human

IHC (paraffin)

Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428â444 (2005).

human

IHC (frozen)

Corver, W. E. et al. Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer. Cytometry 39, 96â107 (2000).

human

FACS

Moll, I., Houdek, P., SchÃ¤fer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437â46 (1999).

human

IHC (paraffin)

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Specifications

Datasheet

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
11414S	anti-Keratin K10 mouse monoclonal, DE-K10	supernatant, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70021	anti-skin layer Keratin sample set		600 µl each antibody	$230.00

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	DE-K10
Immunogen	Cytoskeletal preparation from human epidermis
Purification	hybridoma cell culture supernatant
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note	centrifuge prior to opening
Intended use	Research use only
Tested species reactivity	human

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:5 - 1:20
Immunohistochemistry (IHC) - paraffin	1:5 - 1:20 (protease treatment recommended)
Western Blot (WB)	1:100 - 1:250

Background

Details

MAb DE-K10 specifically recognizes the 56.6 kD keratin K10 (formerly also designated cytokeratin 10) polypeptide in squamous cells and carcinomas from e.g. epidermis, lung, bladder, cervix, oesophagus.
Positive control: Squamous cell carcinoma.

Reference

Publication

Species

Application

Boink, M. et al. Comparison of advanced therapy medicinal product gingiva and skin substitutes and their in vitro wound healing potentials. J.Tissue.Eng.Regen.Med. 12, e1088-e1097 (2018).

human

IHC (paraffin)

Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017).

human

IHC-IF (paraffin)

Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014).

mouse

IHC (frozen)

human
mouse

IHC (frozen)

mouse

IHC (frozen)

Boehnke, K. et al. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur. J. Cell Biol. 86, 731â746 (2007).

human

IHC (paraffin)

Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428â444 (2005).

human

IHC (frozen)

Corver, W. E. et al. Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer. Cytometry 39, 96â107 (2000).

human

FACS

human

IHC (paraffin)

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anti-Keratin K10 mouse monoclonal, DE-K10, supernatant

Alternative Products