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human tongue (courtesy of L. Langbein) Zoom

human tongue (courtesy of L. Langbein)

anti-Keratin K13 guinea pig polyclonal, serum

Excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin.
Cat. No.: GP-K13
Quantity:  100 µL
No. of experiments:  ≤ 400 (IHC, 50 µl/slide); ≤ 60 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$328.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Synthetic peptide (TTTSSASV TTTSNA-C) of human keratin K13 (formerly also designated cytokeratin 13), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide); ≤ 60 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:50 - 1:200
Immunohistochemistry (IHC) - paraffin 1:50 - 1:200 (microwave treatment recommended)
Western Blot (WB) 1:3000

Background

Details

Excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin. Negative on foot sole, scalp.
Reactive polypeptide: Acidic human keratin K13 (Mr 55 000).

Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngiom.

Positive control: Squamous epithelium of esophagus, gingival, palate, vagina (staining all of the suprabasal layers).

Reference

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