Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
65007 |
anti-Keratin K13 mouse monoclonal, Ks13.1 |
prediluted, purified, ready-to-use | 5 mL |
$161.00
Excl. VAT, excl. Shipping Cost
|
19007 |
anti-Keratin K13 mouse monoclonal, Ks13.1 |
prediluted, purified | 1 mL |
$215.00
Excl. VAT, excl. Shipping Cost
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | Ks13.1 |
Immunogen | Keratin K13 of Mr 54 000 purified from human esophagus |
Purification | protein A affinity chromatography |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume) |
Intended use | Research use only |
Tested species reactivity | bovine, human, rat |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:10 |
Immunohistochemistry (IHC) - paraffin | 1:10 (protease treatment and/or microwave treatment recommended) |
Western Blot (WB) | 1:50 - 1:100 |
Background
Details
Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin.
Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13; with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000 and also to K25, formerly designated K25irs1 from inner root sheath of hair follicle).
Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma
Reactivity on cultured cell lines: Cell lines from squamous cell CA, e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder CA
Reference
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