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anti-Keratin K13 mouse monoclonal, Ks13.1, prediluted, purified

The anti-keratin K13 Ks13.1 antibody reacts with non-cornified squamous epithelia and neoplasms derived therefrom. Protease pretreatment improves the performance of this excellent marker in immunohistochemistry.
Cat. No.: 65007
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€129.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks13.1
Immunogen Keratin K13 of Mr 54.000 purified from human esophagus
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin.
Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13; with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000 and also to K25, formerly designated K25irs1 from inner root sheath of hair follicle).
Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma

Reactivity on cultured cell lines: Cell lines from squamous cell CA, e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder

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