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human scalp (courtesy of L. Langbein) Zoom

human scalp (courtesy of L. Langbein)

anti-Keratin K14 guinea pig polyclonal, serum

The antibody reacts with keratin K14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. Correlation between the expression of K14 and prognosis has been discussed.
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Cat. No.: GP-CK14
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€288.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
GP-CK14S

anti-Keratin K14 guinea pig polyclonal

serum, sample (ready-to-use) 600 µL (ready-to-use)
€47.00
Excl. 19% Tax, excl. Shipping Cost
70021

anti-skin layer Keratin sample set

600 µl each antibody
€184.00

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen peptide against human K14 C-VSTHEQVLRTKN (460-472)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 – 1:200
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) 1:5,000 – 1:10,000

Background

Details

Reactive Polypeptide: Acidic keratin K14 (Mr 50 000; formerly also designated cytokeratin 14), expressed in basal and first suprabasal layers of epidermis
Tested cultured cell lines: BPH-1 (derived from non-neoplastic prostatic tissue)
Tumors Specifically Detected: The antiserum reacts with keratin K14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin K14 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references).

Reference

Reference

PublicationSpeciesApplication
Yosef, R. et al. Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL. Nat. Commun. 7, (2016). mouse IHC (paraffin)
Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015). human WB, IHC (paraffin)
Cui, C.-Y. et al. Involvement of Wnt, Eda and Shh at defined stages of sweat gland development. Development 141, 3752–60 (2014). mouse IHC (paraffin)
Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014). mouse IHC (frozen)
Tokarsky-Amiel, R. et al. Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis. Cancer Res. 73, 2829–2839 (2013). mouse IHC (paraffin)
Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750–1758 (2012). mouse IHC (frozen)
Cui, C.-Y. et al. Forkhead transcription factor FoxA1 regulates sweat secretion through Bestrophin 2 anion channel and Na-K-Cl cotransporter 1. Proc. Natl. Acad. Sci. United States Am.  109, 1199–1203 (2012). mouse IHC (paraffin)
Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55–73 (2010). human IHC (frozen)
Jennemann, R. et al. Integrity and Barrier Function of the Epidermis Critically Depend on Glucosylceramide Synthesis. J. Biol. Chem. 282, 3083–3094 (2006). mouse IHC (paraffin)
Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761–774 (2005). human IHC (frozen)
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Langbein, L. et al. Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur. J. Cell Biol. 82, 385–400 (2003). human, bovine IHC (frozen)
Herrmann, T. et al. Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. J. Cell Biol. 161, 1105–1115 (2003). mouse IHC (paraffin)

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