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human oral mucosa (courtesy of J.Heß, University Hospital Heidelberg) Zoom

human oral mucosa (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K15 guinea pig polyclonal, serum

The antibody stains keratin K15 in basal cells of stratified and complex stratified epithelia and in luminal epithelia (mammary, sweat and salivary glands). Found in squamous skin and lung carcinomas and a subgroup of invasive ductal breast carcinomas.
Cat. No.: GP-K15
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€249.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide of human keratin K15 (formerly also designated cytokeratin 15; SNFHINVEESVDGQ-C), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse
Buffer Compatibility Guinea pig polyclonal (GPK15.2)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:200 - 1:500
Immunohistochemistry (IHC) - paraffin 1:200 - 1:500 (protease treatment and/or microwave treatment recommended)

Background

Details

MW 50,000 (pI 4.9) intermediate filament polypeptide, keratin K15, detected by immunohistochemistry in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. broncheal epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the basal cell layer of the outer root sheath.
Completely non-reactive were cells of liver, colon.

Positive Control: Sweat glands of human skin (e.g. foot sole).

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