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human tongue (courtesy of L. Langbein) Zoom

human tongue (courtesy of L. Langbein)

anti-Keratin K15 guinea pig polyclonal, serum

The antibody stains keratin K15 in basal cells of stratified and complex stratified epithelia and in luminal epithelia (mammary, sweat and salivary glands). Found in squamous skin and lung carcinomas and a subgroup of invasive ductal breast carcinomas.
Cat. No.: GP-CK15
Quantity:  100 µL

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€249.00
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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen C-terminal tail region (including parts of the rod domain) of human recombinant keratin K15
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100 - 1:200
Western Blot (WB) 1:2,000

Background

Details

Mr 50 kD (pI 4.9) intermediate filament polypeptide, keratin K15 (formerly also designated cytokeratin 15), detected by immunofluorescence microscopy in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. broncheal epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the outermost cell layer of the outer root sheath; within the hair follicle several cell layers of the hair follicle bulb were positive with antiserum gp 15.1.

In human fetal tissues keratin K15 was localized in basal cells of epidermis, lung, trachea, tracheal glands and esophagus. In fetal hair anlagen, all cells were positive with antiserum gp 15.1, suggesting that keratin K15 is preferentially expressed in epithelial stem cells. Completely unreactive were cells of liver, colon, kidney, urinary bladder, pancreas and lung alveolus.

In tumors, gp15.1 is reactive with squamous skin and lung carcinomas and with a significant subgroup of invasive ductal breast carcinomas, especially tumors with higher grade malignancy.

Reactivity on cultured cell lines: HaCat, A-431; negative on PLC.

Reference

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