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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide of human keratin K16 (formerly also designated cytokeratin 16; C-KE QSS SSF SQG QSS), coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100 – 1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100 – 1:200 (microwave treatment recommended) |
Background
Details
MW 48,000 (pI 5.1) intermediate filament polypeptide, keratin K16, detected by immunohistochemistry in psoriatic skin, in the rete ridges of foot sole epidermis, in tongue, esophagus, exocervix and in squamous cell carcinoma of skin, tongue and cervix. In the hair follicle, staining was restricted to the outer root sheath.
Completely non-reactive were cells of non-stratified epithelia (e.g. liver, colon).
Positive control: Rete ridges of human foot sole epidermis, psoriatic skin.
Reference
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Species
Application
human
IHC-IF (paraffin)
