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IHC of mouse tail (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of mouse tail (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K17 mouse monoclonal, Ks17.E3, lyophilized, purified

The antibody recognizes myoepithelial cells; benign tumors of mammary gland; malignant forms are negative.
highly_published external_validation
Cat. No.: 61036
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$251.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone Ks17.E3
Immunogen human keratin K17 of 46 kDa
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity human, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10 - 1:50
Immunohistochemistry (IHC) - paraffin 1:10 - 1:50 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 17.E3 represents an excellent marker to distinguish myoepithelial cells (positive for K17) from luminal epithelium of various glands (mammary, sweat, salivary, bronchial, tracheal, laryngeal, esophageal) and benign from malignant forms of e.g. mammary gland tumors.
In the epidermis suprabasal staining is found only in cornifying regions and in the outer root sheath of hair follicles.
Tested reactivity on cultured cell lines: HeLa, A-431
Tumors specifically detected: Benign tumors of mammary gland; most malignant forms of mammary tumors are negative for keratin K17

Reference

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