PROGEN - passion for research

close×

anti-Keratin K17 mouse monoclonal, Ks17.E3, supernatant

The antibody recognizes myoepithelial cells; benign tumors of mammary gland; malignant forms are negative.
Cat. No.: 65136
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone Ks17.E3
Immunogen Human keratin K17 of Mr 46,000
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) ready-to-use
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 17.E3 represents an excellent marker to distinguish myoepithelial cells (positive for K17) from luminal epithelium of various glands (mammary, sweat, salivary, bronchial, tracheal, laryngeal, esophageal) and benign from malignant forms of e.g. mammary gland tumors.
In the epidermis suprabasal staining is found only in cornifying regions and in the outer root sheath of hair follicles.
Reactivity on cultured cell lines: HeLa, A-431 
Tumors specifically detected: Benign tumors of mammary gland; most malignant forms of mammary tumors are negative for keratin K17

Reference

Reference

PublicationSpeciesApplication
Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55–73 (2010). human IHC (frozen)
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005). human IHC (paraffin)
Romih, R., Jezernik, K. & Masera, A. Uroplakins and cytokeratins in the regenerating rat urothelium after sodium saccharin treatment. Histochem. Cell Biol. 109, 263–9 (1998). rat IHC (paraffin)
Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518–35 (1995). human IHC (paraffin)
Möbus, V. J. et al. Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis. Br. J. Cancer 69, 422–428 (1994). human ICC-IF
Moll, R. et al. The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation. 53, 75–93 (1993). human IHC (frozen)
Smedts, F. et al. Keratin Expression in Cervical Cancer. Am. J. Pathol. 141, 497–511 (1992). human IHC (frozen)
Smedts, F. et al. Basal-cell Keratins in Cervical Reserve Cells and a Comparison to Their Expression in Cervical Intraepithelial Neoplasia. Am. J. Pathol. 140, (1992). human WB, IHC (frozen)
Wetzels, R. H. et al. Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18. Histopathology 20, 295–303 (1992). human IHC (frozen)
Wetzels, R. H. W. et al. Basal Cell-specific and Hyperproliferation-related Keratins in Human Breast Cancer. Am. J. Pathol. 138, 751–763 (1991). human IHC (frozen)
de Jong, E. M. et al. Keratin 17: a useful marker in anti-psoriatic therapies. Arch. Dermatol. Res. 283, 480–2 (1991). human IHC (frozen)
Moll, I. & Moll, R. Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch. Dermatol. Res. 283, 300–9 (1991). human IHC (frozen)
Troyanovsky, S. M., Guelstein, V. I., Tchipysheva, T. A., Krutovskikh, V. A. & Bannikov, G. A. Patterns of expression of keratin 17 in human epithelia: dependency on cell position. J. Cell Sci. 93, 419–26 (1989). human WB, IHC (frozen), ICC-IF
Guelstein, V. I. et al. Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer. Int. J. cancer 42, 147–53 (1988). human WB, IHC (frozen)

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields