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human kidney (courtesy of J.Heß, University Hospital Heidelberg) Zoom

human kidney (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K18 guinea pig polyclonal, serum

The antiserum represents an excellent marker for all simple (non-stratified) epithelia and basal cells of many squamous, non-cornified epithelia.
Cat. No.: GP-K18
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€249.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide from human keratin K18 (CSYGARPVSSAASVY)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:50 - 1:200
Immunohistochemistry (IHC) - paraffin 1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Reactive polypeptide (specificity): Human type I (acidic) keratin K18 (Mr 45,500; formerly also designated cytokeratin 18)

Tissues specifically detected: The antiserum represents an excellent marker for all simple (non-stratified) epithelia and basal cells of many squamous, non-cornified epithelia. No  cross-reaction with other keratins and intermediate filament proteins.

Tumors specifically detected: All adeno-Ca tested, undifferentiated CA, hepatocellular CA, cervix Ca.

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