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anti-Keratin K18 mouse monoclonal, Ks18.04, Biotin Conjugate

The anti-Keratin K18 Biotin antibody decorates all simple epithelia and epithelium-derived neoplasms. For best performance, Microwave treatment is recommended.
Cat. No.: 61528
Quantity:  250 µL

Delivery Time: usually 1-7 working days

€262.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.04
Immunogen Human keratin K18 from HeLa cytoskeletal preparation
Purification protein A affinity chromatography
Conjugate biotin
Formulation contains 0.09% sodium azide
Storage store at 2 – 8 °C
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, dog, hamster, human, mouse, pig, rat, sheep, trout

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Reactivity on cultured cell lines: MCF-7

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