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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| 61028 |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
lyophilized, purified | 50 µg |
$304.00
Excl. 19% Tax, excl. Shipping Cost
|
| 61528 |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
Biotin Conjugate | 250 µL |
$327.00
Excl. 19% Tax, excl. Shipping Cost
|
| 65028 |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
prediluted, purified | 5 mL |
$191.00
Excl. 19% Tax, excl. Shipping Cost
|
| 61028S |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
purified, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. 19% Tax, excl. Shipping Cost
|
| 70020 |
anti-Keratin sample set |
600 µl each antibody |
$230.00
|
|
| 70023 |
anti-Keratin K8/K18 sample set |
600 µl each antibody |
$105.00
|
|
| 61028-WBC |
anti-Keratin K18, Ks18.04 + positive western blot control |
50 µg anti-Keratin K18 + 50 µg western blot control |
$289.00
|
|
| 690028 |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
liquid, purified | 1 mL (50 µg/mL) |
$425.00
Excl. 19% Tax, excl. Shipping Cost
|
| 690028S |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
liquid, purified, small | 0.2 mL (50 µg/mL) |
$170.00
Excl. 19% Tax, excl. Shipping Cost
|
Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | Ks18.04 |
| Immunogen | human keratin K18 from HeLa cytoskeletal preparation |
| Purification | protein A affinity chromatography |
| Conjugate | FITC |
| Formulation | contains 0.09% sodium azide |
| Storage | store at 2 – 8 °C |
| UniprotID | P05783 |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | bovine, dog, hamster, human, mouse, pig, rat, sheep, trout |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Flow Cytometry (FACS) | assay dependent |
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:10 (microwave treatment recommended) |
Background
Details
Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Tested cultured cell lines: MCF-7
Reference
Reference
- Ikenouchi, J., Matsuda, M., Furuse, M. & Tsukita, S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J. Cell Sci. 116, 1959–67 (2003).
- Stumptner, C., Fuchsbichler, A., Heid, H., Zatloukal, K. & Denk, H. Mallory body--a disease-associated type of sequestosome. Hepatol. 35, 1053–62 (2002).
- BANTEL, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230–239 (2001).
- Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron 14, 29–41 (1995).
- Bártek, J. et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J. Pathol. 164, 215–24 (1991).
- Lauerová, L., Kovarik, J., Bártek, J., Rejthar, A. & Vojtĕsek, B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7, 495–504 (1988).
- Moll, R., Franke, W. W., Schiller, D. L., Geiger, B. & Krepler, R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11–24 (1982).
