anti-Keratin K18 mouse monoclonal, Ks18.04, FITC Conjugate

The antibody decorates all simple epithelia and epithelium-derived neoplasms.

Cat. No.: 61428
Quantity: 250 µL
No. of experiments: ≤ 50 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$342.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Ikenouchi, J., Matsuda, M., Furuse, M. & Tsukita, S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J. Cell Sci. 116, 1959â1967 (2003).

mouse
human

WB
ICC-IF

Stumptner, C. Mallory bodyâA disease-associated type of sequestosome. Hepatology 35, 1053â1062 (2002).

mouse
human

IHC (frozen)
ICC-IF
IEM

Bantel, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230â239 (2001).

human

WB
IHC (paraffin)
ICC

Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron 14, 29â41 (1995).

mouse

IHC (paraffin)

BÃ¡rtek, J. et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J. Pathol. 164, 215â24 (1991).

mouse

WB
IHC (frozen)

LauerovÃ¡, L., Kovarik, J., BÃ¡rtek, J., Rejthar, A. & VojtÄsek, B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7, 495â504 (1988).

Moll, R., Franke, W. W., Schiller, D. L., Geiger, B. & Krepler, R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11â24 (1982).

human

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61028	anti-Keratin K18 mouse monoclonal, Ks18.04	lyophilized, purified	50 µg	$304.00 Excl. VAT, excl. Shipping Cost
61528	anti-Keratin K18 mouse monoclonal, Ks18.04	Biotin Conjugate	250 µL	$327.00 Excl. VAT, excl. Shipping Cost
65028	anti-Keratin K18 mouse monoclonal, Ks18.04	prediluted, purified	5 mL	$191.00 Excl. VAT, excl. Shipping Cost
61028S	anti-Keratin K18 mouse monoclonal, Ks18.04	purified, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70020	anti-Keratin sample set		600 µl each antibody	$230.00
70023	anti-Keratin K8/K18 sample set		600 µl each antibody	$105.00
61028-WBC	anti-Keratin K18, Ks18.04 + positive western blot control		50 µg anti-Keratin K18 + 50 µg western blot control	$289.00
690028	anti-Keratin K18 mouse monoclonal, Ks18.04	liquid, purified	1 mL (50 µg/mL)	$425.00 Excl. VAT, excl. Shipping Cost
690028S	anti-Keratin K18 mouse monoclonal, Ks18.04	liquid, purified, small	0.2 mL (50 µg/mL)	$170.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	Ks18.04
Immunogen	human keratin K18 from HeLa cytoskeletal preparation
Purification	protein A affinity chromatography
Conjugate	FITC
Formulation	contains 0.09% sodium azide
Storage	store at 2-8°C
UniprotID	P05783
No. of experiments	≤ 50 (IHC, 50 µl/slide)
Note	centrifuge prior to opening
Intended use	Research use only
Tested species reactivity	bovine, dog, hamster, human, mouse, pig, rat, sheep, trout

Applications

Tested applications	Tested dilutions
Flow Cytometry (FACS)	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	1:10
Immunohistochemistry (IHC) - paraffin	1:10 (microwave treatment recommended)

Background

Details

Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.

Tested cultured cell lines: MCF-7

Reference

Publication

Species

Application

mouse
human

WB
ICC-IF

Stumptner, C. Mallory bodyâA disease-associated type of sequestosome. Hepatology 35, 1053â1062 (2002).

mouse
human

IHC (frozen)
ICC-IF
IEM

Bantel, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230â239 (2001).

human

WB
IHC (paraffin)
ICC

Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron 14, 29â41 (1995).

mouse

IHC (paraffin)

BÃ¡rtek, J. et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J. Pathol. 164, 215â24 (1991).

mouse

WB
IHC (frozen)

LauerovÃ¡, L., Kovarik, J., BÃ¡rtek, J., Rejthar, A. & VojtÄsek, B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7, 495â504 (1988).

Moll, R., Franke, W. W., Schiller, D. L., Geiger, B. & Krepler, R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11â24 (1982).

human

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anti-Keratin K18 mouse monoclonal, Ks18.04, FITC Conjugate

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