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IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K18 mouse monoclonal, Ks18.04, lyophilized, purified

The anti-keratin K18 antibody decorates all simple epithelia and epithelium-derived neoplasms. For best performance, microwave treatment is recommended.
application_confirmed highly_published external_validation validated_by_epitope_mapping
Cat. No.: 61028
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€221.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.04
Immunogen human keratin K18 from HeLa cytoskeletal preparation
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID P05783
Intended use Research use only
Tested species reactivity bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:20 -1:200
Immunohistochemistry (IHC) - paraffin 1:20 -1:200 (microwave treatment recommended)
Western Blot (WB) 1:100 - 1:500 (0.5 µg/ml - 0.1 µg/ml)

Background

Details

Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.

Tested cultured cell lines: MCF-7

Reference

Reference

PublicationSpeciesApplication
Soady, K. et al. The receptor protein tyrosine phosphatase PTPRB negatively regulates FGF2-dependent branching morphogenesis. Development. 144, 3777-3788 (2017). mouse FACS
Tanaka, J. et al. Generation of orthotopically functional salivary gland from embryonic stem cells. Nat.Commun. 9, 4216 (2018). mouse IHC-IF (frozen)
Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017). human IHC-IF (paraffin)
Soady, K. J. et al. Mouse mammary stem cells express prognostic markers for triple-negative breast cancer. Breast Cancer Res. 17, (2015). mouse ICC-IF
Lokody, I. B., Francis, J. C., Gardiner, J. R., Erler, J. T. & Swain, A. Pten Regulates Epithelial Cytodifferentiation during Prostate Development. PLoS One 10, (2015). mouse IHC (paraffin)
Zatloukal, B. et al. Sensitivity and Specificity of In situ Proximity Ligation for Protein Interaction Analysis in a Model of Steatohepatitis with Mallory-Denk Bodies. PLoS One 9, (2014). mouse IHC (frozen)
Eberle, J. et al. Receptors for short-chain fatty acids in brush cells at the "gastric groove". Front. Physiol. 5, (2014). mouse IHC (frozen)
Foth, M. et al. Fibroblast Growth Factor Receptor 3 activation plays a causative role in urothelial cancer pathogenesis in cooperation with Pten loss in mice. J. Pathol. 233, 148–158 (2014). mouse IHC (paraffin)
Eberle, J. et al. Band-like arrangement of taste-like sensory cells at the gastric groove: evidence for paracrine communication. Front. Physiol. 4, (2013). mouse IHC (frozen)
Eberle, J. et al. Putative interaction of brush cells with bicarbonate secreting cells in the proximal corpus mucosa. Front. Physiol. 4, (2013). mouse IHC (frozen)
Teissedre, B. et al. MMTV-Wnt1 and -DN89b-Catenin Induce Canonical Signaling in Distinct Progenitors and Differentially Activate Hedgehog Signaling within Mammary Tumors. PLoS One 4, (2009). mouse IHC (paraffin)
Campard, D., Lysy, P. A., Najimi, M. & Sokal, E. M. Native Umbilical Cord Matrix Stem Cells Express Hepatic Markers and Differentiate Into Hepatocyte-like Cells. Gastroenterology 134, (2008). human ICC-IF
Nevado, C., Valverde, A. M. & Benito, M. Role of Insulin Receptor in the Regulation of Glucose Uptake in Neonatal Hepatocytes. Endocrinology 147, 3709–3718 (2006). mouse ICC-IF
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005). human IHC (paraffin)
Ikenouchi, J., Matsuda, M., Furuse, M. & Tsukita, S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J. Cell Sci. 116, 1959–1967 (2003). mouse WB, ICC-IF
Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255–263 (2002). human ICC-IF
Stumptner, C. Mallory body—A disease-associated type of sequestosome. Hepatology 35, 1053–1062 (2002). human, mouse IHC (frozen), IEM
Welm, B. E. et al. Sca-1pos Cells in the Mouse Mammary Gland Represent an Enriched Progenitor Cell Population. Dev. Biol. 245, 42–56 (2002). mouse IHC (paraffin)
Bantel, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230–239 (2001). human WB, IHC (paraffin), ICC

 

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