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IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K18 mouse monoclonal, Ks18.04, lyophilized, purified

The anti-keratin K18 antibody decorates all simple epithelia and epithelium-derived neoplasms. For best performance, microwave treatment is recommended.
application_confirmed highly_published external_validation validated_by_epitope_mapping
Cat. No.: 61028
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$304.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.04
Immunogen human keratin K18 from HeLa cytoskeletal preparation
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID P05783
Intended use Research use only
Tested species reactivity bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:20 -1:200
Immunohistochemistry (IHC) - paraffin 1:20 -1:200 (microwave treatment recommended)
Western Blot (WB) 1:100 - 1:500 (0.5 µg/ml - 0.1 µg/ml)

Background

Details

Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.

Tested cultured cell lines: MCF-7

Reference

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