IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K18 mouse monoclonal, Ks18.04, lyophilized, purified

The anti-keratin K18 antibody decorates all simple epithelia and epithelium-derived neoplasms. For best performance, microwave treatment is recommended.

Cat. No.: 61028
Quantity: 50 µg
No. of experiments: ≤ 4000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$304.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Fougner, C. et al. Claudin-low-like mouse mammary tumors show distinct transcriptomic patterns uncoupled from genomic drivers. Breast.Cancer.Res. 21, 85 (2019)

mouse

IHC (paraffin)

Norum, J. et al. GLI1-induced mammary gland tumours are transplantable and maintain major molecular features. Int.J.Cancer. , (2019)

mouse

IHC (paraffin)

Santoro, A. et al. p53 Loss in Breast Cancer Leads to Myc Activation, Increased Cell Plasticity, and Expression of a Mitotic Signature with Prognostic Value. Cell.Rep. 26, 624-638.e8 (2019)

mouse

IHC (paraffin)

Tanaka, J. et al. Generation of orthotopically functional salivary gland from embryonic stem cells. Nat.Commun. 9, 4216 (2018).

mouse

IHC-IF (frozen)

Soady, K. et al. The receptor protein tyrosine phosphatase PTPRB negatively regulates FGF2-dependent branching morphogenesis. Development. 144, 3777-3788 (2017).

mouse

FACS

Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017).

human

IHC-IF (paraffin)

Soady, K. J. et al. Mouse mammary stem cells express prognostic markers for triple-negative breast cancer. Breast Cancer Res. 17, (2015).

mouse

ICC-IF

Lokody, I. B., Francis, J. C., Gardiner, J. R., Erler, J. T. & Swain, A. Pten Regulates Epithelial Cytodifferentiation during Prostate Development. PLoS One 10, (2015).

mouse

IHC (paraffin)

Zatloukal, B. et al. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies. PLoS One 9, (2014).

mouse

IHC (frozen)

Eberle, J. et al. Receptors for short-chain fatty acids in brush cells at the "gastric groove". Front. Physiol. 5, (2014).

mouse

IHC (frozen)

Foth, M. et al. Fibroblast Growth Factor Receptor 3 activation plays a causative role in urothelial cancer pathogenesis in cooperation with Pten loss in mice. J. Pathol. 233, 148â158 (2014).

mouse

IHC (paraffin)

Eberle, J. et al. Band-like arrangement of taste-like sensory cells at the gastric groove: evidence for paracrine communication. Front. Physiol. 4, (2013).

mouse

IHC (frozen)

Eberle, J. et al. Putative interaction of brush cells with bicarbonate secreting cells in the proximal corpus mucosa. Front. Physiol. 4, (2013).

mouse

IHC (frozen)

Teissedre, B. et al. MMTV-Wnt1 and -DN89b-Catenin Induce Canonical Signaling in Distinct Progenitors and Differentially Activate Hedgehog Signaling within Mammary Tumors. PLoS One 4, (2009).

mouse

IHC (paraffin)

Campard, D., Lysy, P. A., Najimi, M. & Sokal, E. M. Native Umbilical Cord Matrix Stem Cells Express Hepatic Markers and Differentiate Into Hepatocyte-like Cells. Gastroenterology 134, (2008).

human

ICC-IF

Nevado, C., Valverde, A. M. & Benito, M. Role of Insulin Receptor in the Regulation of Glucose Uptake in Neonatal Hepatocytes. Endocrinology 147, 3709â3718 (2006).

mouse

ICC-IF

Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428â444 (2005).

human

IHC (frozen)

Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259â71 (2005).

human

IHC (paraffin)

Ikenouchi, J., Matsuda, M., Furuse, M. & Tsukita, S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J. Cell Sci. 116, 1959â1967 (2003).

mouse

WB
ICC-IF

Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255â262 (2002).

human

ICC-IF

Stumptner, C. Mallory bodyâA disease-associated type of sequestosome. Hepatology 35, 1053â1062 (2002).

human
mouse

IHC (frozen)
IEM

Welm, B. E. et al. Sca-1pos Cells in the Mouse Mammary Gland Represent an Enriched Progenitor Cell Population. Dev. Biol. 245, 42â56 (2002).

mouse

IHC (paraffin)

Bantel, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230â239 (2001).

human

WB
IHC (paraffin)
ICC

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
61528	anti-Keratin K18 mouse monoclonal, Ks18.04	Biotin Conjugate	250 µL	$327.00 Excl. VAT, excl. Shipping Cost
65028	anti-Keratin K18 mouse monoclonal, Ks18.04	prediluted, purified	5 mL	$191.00 Excl. VAT, excl. Shipping Cost
61428	anti-Keratin K18 mouse monoclonal, Ks18.04	FITC Conjugate	250 µL	$342.00 Excl. VAT, excl. Shipping Cost
61028S	anti-Keratin K18 mouse monoclonal, Ks18.04	purified, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70020	anti-Keratin sample set		600 µl each antibody	$230.00
70023	anti-Keratin K8/K18 sample set		600 µl each antibody	$105.00
61028-WBC	anti-Keratin K18, Ks18.04 + positive western blot control		50 µg anti-Keratin K18 + 50 µg western blot control	$289.00
690028	anti-Keratin K18 mouse monoclonal, Ks18.04	liquid, purified	1 mL (50 µg/mL)	$425.00 Excl. VAT, excl. Shipping Cost
690028S	anti-Keratin K18 mouse monoclonal, Ks18.04	liquid, purified, small	0.2 mL (50 µg/mL)	$170.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	Ks18.04
Immunogen	human keratin K18 from HeLa cytoskeletal preparation
Purification	protein A affinity chromatography
Conjugate	unconjugated
Formulation	lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID	P05783
No. of experiments	≤ 4000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)
Intended use	Research use only
Tested species reactivity	bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	1:20 -1:200
Immunohistochemistry (IHC) - paraffin	1:20 -1:200 (microwave treatment recommended)
Western Blot (WB)	1:100 - 1:500 (0.5 µg/ml - 0.1 µg/ml)

Background

Details

Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.

Tested cultured cell lines: MCF-7

Reference

Publication

Species

Application

Fougner, C. et al. Claudin-low-like mouse mammary tumors show distinct transcriptomic patterns uncoupled from genomic drivers. Breast.Cancer.Res. 21, 85 (2019)

mouse

IHC (paraffin)

Norum, J. et al. GLI1-induced mammary gland tumours are transplantable and maintain major molecular features. Int.J.Cancer. , (2019)

mouse

IHC (paraffin)

Santoro, A. et al. p53 Loss in Breast Cancer Leads to Myc Activation, Increased Cell Plasticity, and Expression of a Mitotic Signature with Prognostic Value. Cell.Rep. 26, 624-638.e8 (2019)

mouse

IHC (paraffin)

Tanaka, J. et al. Generation of orthotopically functional salivary gland from embryonic stem cells. Nat.Commun. 9, 4216 (2018).

mouse

IHC-IF (frozen)

Soady, K. et al. The receptor protein tyrosine phosphatase PTPRB negatively regulates FGF2-dependent branching morphogenesis. Development. 144, 3777-3788 (2017).

mouse

FACS

Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017).

human

IHC-IF (paraffin)

Soady, K. J. et al. Mouse mammary stem cells express prognostic markers for triple-negative breast cancer. Breast Cancer Res. 17, (2015).

mouse

ICC-IF

Lokody, I. B., Francis, J. C., Gardiner, J. R., Erler, J. T. & Swain, A. Pten Regulates Epithelial Cytodifferentiation during Prostate Development. PLoS One 10, (2015).

mouse

IHC (paraffin)

Zatloukal, B. et al. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies. PLoS One 9, (2014).

mouse

IHC (frozen)

Eberle, J. et al. Receptors for short-chain fatty acids in brush cells at the "gastric groove". Front. Physiol. 5, (2014).

mouse

IHC (frozen)

Foth, M. et al. Fibroblast Growth Factor Receptor 3 activation plays a causative role in urothelial cancer pathogenesis in cooperation with Pten loss in mice. J. Pathol. 233, 148â158 (2014).

mouse

IHC (paraffin)

Eberle, J. et al. Band-like arrangement of taste-like sensory cells at the gastric groove: evidence for paracrine communication. Front. Physiol. 4, (2013).

mouse

IHC (frozen)

Eberle, J. et al. Putative interaction of brush cells with bicarbonate secreting cells in the proximal corpus mucosa. Front. Physiol. 4, (2013).

mouse

IHC (frozen)

Teissedre, B. et al. MMTV-Wnt1 and -DN89b-Catenin Induce Canonical Signaling in Distinct Progenitors and Differentially Activate Hedgehog Signaling within Mammary Tumors. PLoS One 4, (2009).

mouse

IHC (paraffin)

Campard, D., Lysy, P. A., Najimi, M. & Sokal, E. M. Native Umbilical Cord Matrix Stem Cells Express Hepatic Markers and Differentiate Into Hepatocyte-like Cells. Gastroenterology 134, (2008).

human

ICC-IF

Nevado, C., Valverde, A. M. & Benito, M. Role of Insulin Receptor in the Regulation of Glucose Uptake in Neonatal Hepatocytes. Endocrinology 147, 3709â3718 (2006).

mouse

ICC-IF

Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428â444 (2005).

human

IHC (frozen)

Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259â71 (2005).

human

IHC (paraffin)

mouse

WB
ICC-IF

Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255â262 (2002).

human

ICC-IF

Stumptner, C. Mallory bodyâA disease-associated type of sequestosome. Hepatology 35, 1053â1062 (2002).

human
mouse

IHC (frozen)
IEM

Welm, B. E. et al. Sca-1pos Cells in the Mouse Mammary Gland Represent an Enriched Progenitor Cell Population. Dev. Biol. 245, 42â56 (2002).

mouse

IHC (paraffin)

Bantel, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230â239 (2001).

human

WB
IHC (paraffin)
ICC

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anti-Keratin K18 mouse monoclonal, Ks18.04, lyophilized, purified

Alternative Products