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Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
61528 |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
Biotin Conjugate | 250 µL |
$327.00
Excl. VAT, excl. Shipping Cost
|
65028 |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
prediluted, purified | 5 mL |
$191.00
Excl. VAT, excl. Shipping Cost
|
61428 |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
FITC Conjugate | 250 µL |
$342.00
Excl. VAT, excl. Shipping Cost
|
61028S |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
purified, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
70020 |
anti-Keratin sample set |
600 µl each antibody |
$230.00
|
|
70023 |
anti-Keratin K8/K18 sample set |
600 µl each antibody |
$105.00
|
|
61028-WBC |
anti-Keratin K18, Ks18.04 + positive western blot control |
50 µg anti-Keratin K18 + 50 µg western blot control |
$289.00
|
|
690028 |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
liquid, purified | 1 mL (50 µg/mL) |
$425.00
Excl. VAT, excl. Shipping Cost
|
690028S |
anti-Keratin K18 mouse monoclonal, Ks18.04 |
liquid, purified, small | 0.2 mL (50 µg/mL) |
$170.00
Excl. VAT, excl. Shipping Cost
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | Ks18.04 |
Immunogen | human keratin K18 from HeLa cytoskeletal preparation |
Purification | protein A affinity chromatography |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
UniprotID | P05783 |
No. of experiments | ≤ 4000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume) |
Intended use | Research use only |
Tested species reactivity | bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:20 -1:200 |
Immunohistochemistry (IHC) - paraffin | 1:20 -1:200 (microwave treatment recommended) |
Western Blot (WB) | 1:100 - 1:500 (0.5 µg/ml - 0.1 µg/ml) |
Background
Details
Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Tested cultured cell lines: MCF-7
Reference
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