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anti-Keratin K18 mouse monoclonal, Ks18.04, prediluted, purified

The anti-keratin K18 antibody decorates all simple epithelia and epithelium-derived neoplasms. For best performance, Microwave treatment is recommended.
Cat. No.: 65028
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.04
Immunogen human keratin K18 from HeLa cytoskeletal preparation
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Reactivity on cultured cell lines: HeLa and MCF-7

Reference

Reference

  1. Anderson, L. H., Boulanger, C. A., Smith, G. H., Carmeliet, P. & Watson, C. J. Stem cell marker Prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland. Dev Dyn.; Author Manuscr. 240, 674–681 (2012).
  2. Thomsen, M. K., Butler, C. M., Shen, M. M. & Swain, A. Sox9 is required for prostate development. Dev. Biol. 316, 302–311 (2008).
  3. Schaffeld, M., Knappe, M., Hunzinger, C. & Markl, J. cDNA sequences of the authentic keratins 8 and 18 in zebrafish. Differentiation. 71, 73–82 (2003).
  4. Stumptner, C., Fuchsbichler, A., Heid, H., Zatloukal, K. & Denk, H. Mallory body - a disease-associated type of sequestosome. Hepatol.  35, 1053–62 (2002).
  5. Bantel, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230–239 (2001).
  6. Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron 14, 29–41 (1995).
  7. Bártek, J. et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J. Pathol. 164, 215–24 (1991).
  8. Lauerová, L., Kovarik, J., Bártek, J., Rejthar, A. & Vojtĕsek, B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7, 495–504 (1988).
  9. Moll, R., Franke, W. W., Schiller, D. L., Geiger, B. & Krepler, R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11–24 (1982).

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