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anti-Keratin K18 mouse monoclonal, Ks18.04, prediluted, purified

The anti-keratin K18 antibody decorates all simple epithelia and epithelium-derived neoplasms. For best performance, Microwave treatment is recommended.
Cat. No.: 65028
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.04
Immunogen human keratin K18 from HeLa cytoskeletal preparation
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Reactivity on cultured cell lines: HeLa and MCF-7

Reference

Reference

PublicationSpeciesApplication
Zatloukal, B. et al. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies. PLoS One 9, (2014). mouse IHC (frozen)
Anderson, L. H., Boulanger, C. A., Smith, G. H., Carmeliet, P. & Watson, C. J. Stem cell marker Prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland. Dev Dyn.; Author Manuscr. 240, 674–681 (2012). mouse IHC (frozen)
Thomsen, M. K., Butler, C. M., Shen, M. M. & Swain, A. Sox9 is required for prostate development. Dev. Biol. 316, 302–311 (2008). mouse IHC (paraffin)
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005). human IHC (paraffin)
Ikenouchi, J., Matsuda, M., Furuse, M. & Tsukita, S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J. Cell Sci. 116, 1959–1967 (2003). mouse WB, ICC-IF
Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255–263 (2002). human ICC-IF
Stumptner, C., Fuchsbichler, A., Heid, H., Zatloukal, K. & Denk, H. Mallory body - a disease-associated type of sequestosome. Hepatol.  35, 1053–62 (2002). human, mouse IHC (frozen), IEM
Bantel, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230–239 (2001). human WB, IHC (paraffin), ICC

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