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anti-Keratin K18 mouse monoclonal, Ks18.04, prediluted, purified

The anti-keratin K18 antibody decorates all simple epithelia and epithelium-derived neoplasms. For best performance, Microwave treatment is recommended.
Cat. No.: 65028
Quantity:  5 mL

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.04
Immunogen human keratin K18 from HeLa cytoskeletal preparation
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent



Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Reactivity on cultured cell lines: HeLa and MCF-7



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  2. Thomsen, M. K., Butler, C. M., Shen, M. M. & Swain, A. Sox9 is required for prostate development. Dev. Biol. 316, 302–311 (2008).
  3. Schaffeld, M., Knappe, M., Hunzinger, C. & Markl, J. cDNA sequences of the authentic keratins 8 and 18 in zebrafish. Differentiation. 71, 73–82 (2003).
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  7. Bártek, J. et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J. Pathol. 164, 215–24 (1991).
  8. Lauerová, L., Kovarik, J., Bártek, J., Rejthar, A. & Vojtĕsek, B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7, 495–504 (1988).
  9. Moll, R., Franke, W. W., Schiller, D. L., Geiger, B. & Krepler, R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11–24 (1982).

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