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anti-Keratin K18 mouse monoclonal, Ks18.04, supernatant

The anti-Keratin K18 antibody decorates all simple epithelia and epithelium-derived neoplasms. Ready-to-use for immunohistochemistry.

Cat. No.: 65128
Quantity:  5 mL

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.04
Immunogen Human keratin K18 from HeLa cytoskeletal preparation
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Reactivity on cultured cell lines: HeLa and MCF-7

Reference

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